| Cloning and characterization of a new actin gene from Oryza sativa L. |
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| 作者姓名： | LIANG Weihong TANG Chaorong WU Naihu |
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| 作者单位： | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China,Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China,Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China |
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| 基金项目： | 国家高技术研究发展计划(863计划)，the National Basic Science Initiative Fund |
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| 摘 要： | Using Rho family member osRACD as bait, a new member of actin gene family -Act was isolated from Oryza sativa by yeast two-hybrid system. The full-length cDNA was cloned with 5' RACE technology, which contains an open reading frame of 1134 bp with a predicted protein of 377 amino acids. Sequence alignment revealed 96% to 81.8% identities with some known actin proteins in plants. The method of bioinformatics was used to analyze the protein modification sites, structure and evolution of the gene. Southern blot analysis showed that Act is a single-copy gene in the genome. The result of RT-PCR showed it is ubiquitously expressed in root, shoot, callus and panicle in a temporal fashion. The relationship between Rho family and actin family in evolution and function was also studied.
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| 关 键 词： | rice panicle yeast two-hybrid actin Rho gene clone |
Cloning and characterization of a new actin gene from Oryza sativa L. |
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| LIANG Weihong,TANG Chaorong,WU Naihu.Cloning and characterization of a new actin gene from Oryza sativa L.[J].Progress in Natural Science,2004,14(10):867-874. |
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| Authors: | LIANG Weihong TANG Chaorong WU Naihu |
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| Institution: | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China |
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| Abstract: | Using Rho family member osRACD as bait, a new member of actin gene family -Act was isolated from Oryza sativa by yeast two-hybrid system. The full-length cDNA was cloned with 5' RACE technology, which contains an open reading frame of 1134 bp with a predicted protein of 377 amino acids. Sequence alignment revealed 96% to 81.8% identities with some known actin proteins in plants. The method of bioinformatics was used to analyze the protein modification sites, structure and evolution of the gene. Southern blot analysis showed that Act is a single-copy gene in the genome. The result of RT-PCR showed it is ubiquitously expressed in root, shoot, callus and panicle in a temporal fashion. The relationship between Rho family and actin family in evolution and function was also studied. |
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| Keywords: | rice panicle yeast two-hybrid actin Rho gene clone |
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