| 漆酶的层层自组装固定化及其酶学性质 |
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| 引用本文： | 李辉,付时雨,彭林才,詹怀宇.漆酶的层层自组装固定化及其酶学性质[J].华南理工大学学报(自然科学版),2011,39(9):158-164. |
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| 作者姓名： | 李辉 付时雨 彭林才 詹怀宇 |
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| 作者单位： | 华南理工大学制浆造纸工程国家重点实验室,广东广州,510640 |
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| 基金项目： | 国家“973”计划项目(2010BC732206); 国家自然科学基金资助项目(30771689,30972324); 教育部高校博士点基金资助项目(20090172110022) |
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| 摘 要： | 利用静电层层自组装方法对漆酶进行固定化,在固体基底(云母片)上制备了阳离子聚丙烯酰胺( CPAM)/漆酶自组装膜；通过紫外可见吸收光谱、原子力显微镜对自组装膜的活力增长情况及表面形貌进行分析表征,并对层层自组装方法固定化漆酶的酶学性质进行了研究.结果表明:CPAM/漆酶自组装膜的活力随着自组装层数的增多呈线性增长,通过...
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| 关 键 词： | 漆酶 层层自组装 固定化 热稳定性 存储稳定性 |
Immobilization of Laccase via Layer-by-Layer Self-Assembly and Its Enzymatic Properties |
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| Li Hui,Fu Shi-yu,Peng Lin-cai,Zhan Huai-yu.Immobilization of Laccase via Layer-by-Layer Self-Assembly and Its Enzymatic Properties[J].Journal of South China University of Technology(Natural Science Edition),2011,39(9):158-164. |
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| Authors: | Li Hui Fu Shi-yu Peng Lin-cai Zhan Huai-yu |
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| Institution: | Li Hui Fu Shi-yu Peng Lin-cai Zhan Huai-yu(State Key Laboratory of Pulp and Paper Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China) |
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| Abstract: | Laccase was immobilized through the electrostatic layer-by-layer self-assembly,and cationic polyacrylamide(CPAM) /laccase self-assembled films were prepared on solid substrate(mica).Then,the activities and morphologies of the films were characterized by means of the UV-Vis spectroscopy and the atomic force microscopy,and the enzymatic properties of the immobilized laccase were also analyzed.The results show that the activity of the CPAM /laccase self-assembled film linearly increases with the number of laye... |
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| Keywords: | laccase layer-by-layer self-assembly immobilization thermal stability storage stability |
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