| 亚热带木腐真菌产漆酶及其酶学性质 |
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| 引用本文： | 傅恺,付时雨,张丽,周攀登,詹怀宇.亚热带木腐真菌产漆酶及其酶学性质[J].华南理工大学学报(自然科学版),2011,39(9):152-157,164. |
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| 作者姓名： | 傅恺 付时雨 张丽 周攀登 詹怀宇 |
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| 作者单位： | 华南理工大学制浆造纸工程国家重点实验室,广东广州,510640 |
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| 基金项目： | 国家自然科学基金资助项目(30771689); 广东省科技计划项目(2007B031600002) |
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| 摘 要： | 对选育的一株木腐真菌Psathyrella candolleana进行液体深层发酵生产漆酶,通过硫酸铵盐析和离子交换色谱等纯化技术分离发酵酶液后,得到纯度较高的漆酶,并对其酶学性质进行研究.结果表明:P.candolleana在发酵罐中的漆酶产量最高可达9 100 U/L;分离纯化后得到漆酶活性组分,其比活力达到438...
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| 关 键 词： | 白黄小脆柄菇 白腐菌 漆酶 纯化 漆酶活力 稳定性 |
Production and Enzymatic Properties of Laccase from a Subtropical Wood-Rot Fungus |
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| Fu Kai,Fu Shi-yu,Zhang Li,Zhou Pan-deng,Zhan Huai-yu.Production and Enzymatic Properties of Laccase from a Subtropical Wood-Rot Fungus[J].Journal of South China University of Technology(Natural Science Edition),2011,39(9):152-157,164. |
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| Authors: | Fu Kai Fu Shi-yu Zhang Li Zhou Pan-deng Zhan Huai-yu |
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| Institution: | Fu Kai Fu Shi-yu Zhang Li Zhou Pan-deng Zhan Huai-yu(State Key Laboratory of Pulp and Paper Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China) |
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| Abstract: | Laccase from a newly isolated wood-rot fungus Psathyrella Candolleana,was produced via the submerged fermentation,and high-purity laccase was obtained by means of the ammonium sulfate precipitation and the ion-exchange chromatography.Then,the enzymatic properties of the laccase were also analyzed.The results indicate that the maximal activity of the laccase from P.candolleana in the fermentor reaches 9 100 U/L,that the active component of the laccase achieved after 13.2-fold purification is of a specific ac... |
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| Keywords: | Psathyrella Candolleana white-rot fungus laccase purification laccase activity stability |
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