| 木瓜蛋白酶对壳聚糖的降解特性 |
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| 引用本文： | 黄永春,李琳,郭祀远,蔡妙颜.木瓜蛋白酶对壳聚糖的降解特性[J].华南理工大学学报(自然科学版),2003,31(6):71-75. |
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| 作者姓名： | 黄永春 李琳 郭祀远 蔡妙颜 |
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| 作者单位： | 华南理工大学,轻化工研究所,广东,广州,510640 |
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| 基金项目： | 教育部高等学校骨干教师计划资助项目(教技司[2000]65) |
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| 摘 要： | 研究木瓜蛋白酶催化壳聚糖降解过程中，温度、pH值、酶量、底物质量浓度以及脱乙酰度等条件对降解速率的影响，并通过计算机随机模拟试验，求出壳聚糖的脱乙酰度与GlcNAc-G1cN糖苷键相对含量的关系．结果表明，木瓜蛋白酶只选择性地降解GlcNAc-G1cN糖苷键，反应最适pH值和温度分别为4．5和45℃．
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| 关 键 词： | 壳聚糖 木瓜蛋白酶 降解 计算机模拟 |
| 文章编号： | 1000-565(2003)06-0071-05 |
| 修稿时间： | 2002年9月16日 |
Characteristics of Chitosan Degradation by Papain |
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| Huang Yong-chun Li Lin Guo Si-yuan Cai Miao-yan.Characteristics of Chitosan Degradation by Papain[J].Journal of South China University of Technology(Natural Science Edition),2003,31(6):71-75. |
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| Authors: | Huang Yong-chun Li Lin Guo Si-yuan Cai Miao-yan |
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| Abstract: | The effects of reaction temperature, pH, enzyme content, chitosan mass concentration and degree of deacetylation on the chitosan degradation by papain were studied. The relationship between the degree of deacetylation and the relative content of GlcNAc-GlcN glycosidic linkages of chitosan was found by computer simulation. The results showed that papain only degraded the GlcNAc-GlcN glycosidic linkages, and the suitable temperature and pH were 45 t and 4.5, respectively. |
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| Keywords: | chitosan papain degradation computer simulation |
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