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| DNA疫苗经基因枪介导的免疫研究 | |
| 引用本文： | 韩双艳,李杰生,郭勇,杨慧兰,王捷,杨传红,詹纯列.DNA疫苗经基因枪介导的免疫研究[J].华南理工大学学报(自然科学版),2004,32(3):33-36. |
| 作者姓名： | 韩双艳 李杰生 郭勇 杨慧兰 王捷 杨传红 詹纯列 |
| 作者单位： | 1. 华南理工大学,食品与生物工程学院,广东,广州510640 2. 湖南省怀化市解放军五三五医院,检验科,湖南,怀化418008 3. 广州军区广州总医院,皮肤科,广东,广州,510010 4. 广州军区广州总医院,医学实验科,广东,广州,510010 |
| 基金项目： | 国家自然科学基金资助项目 (30 2 71192 ) |
| 摘 要： | 将基因枪介导的DNA疫苗用于小鼠腹部免疫，并用免疫组化法检测gD抗原在接种部位的表达，利用ELISA检测血清中的抗gD抗体，并采用病毒攻击检测DNA疫苗对动物的保护效果。结果表明，基因枪可有效地将DNA质粒运送至小鼠皮肤组织，DNA疫苗携带的抗原保护基因gD2糖蛋白能在真皮和肌肉组织中高效表达。同时，免疫小鼠体内产生高滴度的中和抗体，能有效抵抗HSV2病毒的攻击。 |
| 关 键 词： | 基因枪 基因表达 抗体 DNA疫苗 |
| 文章编号： | 1000-565X(2004)03-0033-04 |
| 修稿时间： | 2003年9月23日 |
DNA Vaccine Immune Mediated by Gene Gun |
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| Han Shuang-yan,Li Jie-sheng,Guo Yong,Yang Hui-lan,Wang Jie,Yang Chuan-hong,Zhan Chun-lie.DNA Vaccine Immune Mediated by Gene Gun[J].Journal of South China University of Technology(Natural Science Edition),2004,32(3):33-36. | |
| Authors: | Han Shuang-yan Li Jie-sheng Guo Yong Yang Hui-lan Wang Jie Yang Chuan-hong Zhan Chun-lie |
| Institution: | Han Shuang-yan 1 Li Jie-sheng 2 Guo Yong 1 Yang Hui-lan 3 Wang Jie 4 Yang Chuan-hong 4 Zhan Chun-lie 4 |
| Abstract: | The DNA vaccine mediated by gene gun was used to vaccinate the abdomen of mice,and immuno-histological examination was applied to the expression of gD antigen at inoculation sites. The induced IgG antibody was assayed with ELISA and the protective effect of DNA vaccine on animals was investigated. The results show that,by the mediation of gene gun,DNA vaccine plasmid can be successfully delivered to the cutis of mice, that the antigen protection gene gD2 glycoprotein can effectively express in the cutis and muscle of mice,and that high titer neutralizing antibody were induced in immunized mice,which will protect the mice against HSV2 virus. |
| Keywords: | gene gun gene expression antibody DNA vaccine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |