gD2 DNA疫苗诱导细胞免疫应答

用生殖器疱疹gD2 DNA疫苗pcDNA3-gD转染哺乳动物细胞COS-7，鉴定gD表达．再用其免疫BALB／c小鼠，测定脾细胞增殖、CTL细胞杀伤活性，以及CD4^ 和CD8^ 细胞群．结果表明：pcDNA3-gD能够在哺乳动物细胞COS-7中表达gD抗原，其免疫小鼠脾细胞增殖活性增加，CTL活性达37．69％，明显高于pcDNA3组和生理盐水组；CD4^ 占T细胞数量的33．38％，数量较pcDNA3组和生理盐水组显著增加．

Cellular Immunity Induced by gD2 DNA Vaccine

pcDNA3-gD, the constructed Genital Herpes gD DNA vaccine (gD2 DNA vaccine), was transfected into mammalian cell COS-7, and the expression of gD gene was investigated. BALB/c mice were then immunized by pcDNA3-gD. The proliferation of spleen cell, the activity of cytotoxic T cell (CTL) and CD4 +and CD8 + populations were respectively analyzed. The results show that pcDNA3-gD plasmid can express the gene of gD in cell COS-7, that the spleen cell of mice immunized by pcDNA3-gD plasmid is of an increased proliferation activity, that the activity of CTL in the experiment group (immunized by pcDNA3-gD plasmid) is 37.69%which is obviously higher than that of the control group (immunized by pcDNA3 plasmid) and the naive group (immunized by normal saline), and that the percentage of CD4 + cells in the experiment group increases to 33.38% which is greater than that of the control group and the naive group.