| 超声作用下的酶促废油脂转酯反应 |
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| 引用本文： | 吴虹,宗敏华,娄文勇,陈志锋.超声作用下的酶促废油脂转酯反应[J].华南理工大学学报(自然科学版),2006,34(5):68-71. |
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| 作者姓名： | 吴虹 宗敏华 娄文勇 陈志锋 |
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| 作者单位： | 华南理工大学,生物工程系,广东,广州,510640 |
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| 基金项目： | 广东省科技厅科技计划;广东省广州市科技计划 |
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| 摘 要： | 探讨了超声对无溶剂体系中固定化脂肪酶Novozym435催化废油脂转酯反应的促进作用，研究了超声功率、超声处理方式等对酶反应速度及酶操作稳定性的影响．结果表明：对酶进行适宜的超声预处理（20kHz，80W，30min）能显著提高Novozym435催化废油脂转酯反应的速度；经超声预处理的Novozym435有良好的操作稳定性，反应10批次后．酶活下降甚微．
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| 关 键 词： | 超声辐照 脂肪酶 废油脂 转酯反应 |
| 文章编号： | 1000-565X（2006）05-0068-04 |
| 收稿时间： | 2005-03-28 |
| 修稿时间： | 2005年3月28日 |
Enzymatic Transesterification of Waste Oil Under Ultrasonic Irradiation |
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| Wu Hong,Zong Min-hua,Lou Wen-yong,Chen Zhi-feng.Enzymatic Transesterification of Waste Oil Under Ultrasonic Irradiation[J].Journal of South China University of Technology(Natural Science Edition),2006,34(5):68-71. |
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| Authors: | Wu Hong Zong Min-hua Lou Wen-yong Chen Zhi-feng |
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| Institution: | Dept. of Biotechnology, South China Univ. of Tech. , Guangzhou 510640, Guangdong, China |
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| Abstract: | Aiming at the Novozym 435-catalyzed transesterification of waste oil in a solvent-free system under ultrasonic irradiation, the effects of ultrasonic power and radiation mode on the reaction rate and the operation stability were investigated. It is found that the reaction rate of Novozym 435-catalyzed transesterification of waste oil is remarkably accelerated by pretreatment of enzyme with ultrasonic irradiation at 20 kHz, 80 W for 30 min, and that Novozym 435 pretreated with ultrasonic irradiation is of good operation stability because its activity weakens little even after 10 batch operations. |
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| Keywords: | ultrasonic irradiation lipase waste oil transesterification |
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