| 发酵液中HA含量分析方法的研究 |
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| 引用本文： | 周荣清,郭祀远,李琳,胡松青.发酵液中HA含量分析方法的研究[J].华南理工大学学报(自然科学版),2001,29(11):55-58. |
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| 作者姓名： | 周荣清 郭祀远 李琳 胡松青 |
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| 作者单位： | 华南理工大学食品与生物工程学院,广东,广州,510640 |
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| 基金项目： | 四川省科委重大攻关项目基金资助 (970 0 72 ) |
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| 摘 要： | 比较了咔唑法、地衣酚法、阿利新蓝法和Bitter-Muir法等4种分光光度法应用于分析透明质酸质量浓度的准确度和精确度，发现Bitter-Muir法适用于测定透明质酸质量浓度；同时探讨了糖分、蛋白质、酵母膏、无机盐等发酵液组成对Bitter-Muir法分析透明质酸质量浓度的影响，结果表明，这些组成对该分析方法有一定程度的干扰。
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| 关 键 词： | 透明质酸 发酵液 分析方法 质量浓度 分光光度法 咔唑法 地衣酚法 |
| 文章编号： | 1000-565X(2001)11-0055-04 |
| 修稿时间： | 2001年3月21日 |
Methods for Analyzing HA Content in the Fermentative Broth |
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| Zhou Rong_qing,Guo Si_yuan,Li Lin,Hu Song_qing.Methods for Analyzing HA Content in the Fermentative Broth[J].Journal of South China University of Technology(Natural Science Edition),2001,29(11):55-58. |
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| Authors: | Zhou Rong_qing Guo Si_yuan Li Lin Hu Song_qing |
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| Abstract: | Four spectrophotometries such as Carbazole method, Oreinol method, Alcian method and Bitter_Muir method, were adopted to analyze HA mass concentration and their accuracy and precision. The Bitter_Muir method is found applicable to determine HA mass concentration. The affects of impurity in fermentative broth such as resident glucose, protein, yeast and inorganic salt on the Bitter_Muir method for HA analysis were investigated. The result shows that the Bitter_Muir method is interfered by these impurities to some extent. |
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| Keywords: | hyaluronic acid fermentative broth analytical method |
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