微乳层析分离鉴定青梅提取物中的黄酮类成分

研究了一种新的青梅中黄酮类组分微乳薄膜色谱分离鉴定方法．以10％的甲酸调酸、含水量为70％的十二炕基硫酸钠一正丁醇一正庚烷一水微乳液为展开剂，以芦丁为对照样，利用聚酰胺薄膜层析色谱法使青梅黄酮化合物完全分离．分离结果表明，乙酸乙酯组分分离得到5个斑点，氯仿组分分离得到4个斑点，石油醚组分和环己炕组分各分离得到1个斑点．与用正丁醇一乙酸一水为展开剂的常规层析方法相比，微乳薄膜层析法的检测灵敏度更高，分离效果更好。

Separation and Identification of Flavonoids in Extracts of Prunus Mume by Microemulsion Chromatography

This paper proposes a new method for the separation and identification of flavonoids in Prunus mume by microemulsion chromatography. By using 10% formic acid for the pH adjustment, as well as with SDS/n-C_4H_9OH/n-C_7H_ 16 /H_2O microemulsion and rutin respectively as the eluant and the control sample on the polyamide film, the flavonoids were entirely separated by the proposed method. The separated results indicate that five flavonoid spots can be obtained from the ethyl acetate extract, four from chloroform, one from petroleum ether and one from n-hexanel. It is also indicated that, compared with the general chromatography methods with C_4H_9OH/CH_3COOH/H_2O as the eluant, the proposed microemulsion chromatography is of greater sensitivity of detection and better separation effect.