Single mimivirus particles intercepted and imaged with an X-ray laser |
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Authors: | Seibert M Marvin Ekeberg Tomas Maia Filipe R N C Svenda Martin Andreasson Jakob Jönsson Olof Odić Duško Iwan Bianca Rocker Andrea Westphal Daniel Hantke Max DePonte Daniel P Barty Anton Schulz Joachim Gumprecht Lars Coppola Nicola Aquila Andrew Liang Mengning White Thomas A Martin Andrew Caleman Carl Stern Stephan Abergel Chantal Seltzer Virginie Claverie Jean-Michel Bostedt Christoph Bozek John D Boutet Sébastien Miahnahri A Alan Messerschmidt Marc Krzywinski Jacek Williams Garth Hodgson Keith O Bogan Michael J Hampton Christina Y Sierra Raymond G Starodub Dmitri Andersson Inger Bajt Saša |
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Institution: | Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3, SE-751 24 Uppsala, Sweden. |
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Abstract: | X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions. Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma. The continuous diffraction pattern of non-crystalline objects permits oversampling and direct phase retrieval. Here we show that high-quality diffraction data can be obtained with a single X-ray pulse from a non-crystalline biological sample, a single mimivirus particle, which was injected into the pulsed beam of a hard-X-ray free-electron laser, the Linac Coherent Light Source. Calculations indicate that the energy deposited into the virus by the pulse heated the particle to over 100,000?K after the pulse had left the sample. The reconstructed exit wavefront (image) yielded 32-nm full-period resolution in a single exposure and showed no measurable damage. The reconstruction indicates inhomogeneous arrangement of dense material inside the virion. We expect that significantly higher resolutions will be achieved in such experiments with shorter and brighter photon pulses focused to a smaller area. The resolution in such experiments can be further extended for samples available in multiple identical copies. |
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