| 流动分析法测定烟草中蛋白质含量前处理方法的改进 |
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| 引用本文： | 李秋潼,王鹏,尚军,吕祥敏.流动分析法测定烟草中蛋白质含量前处理方法的改进[J].湖南文理学院学报(自然科学版),2012(3):57-59,76. |
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| 作者姓名： | 李秋潼 王鹏 尚军 吕祥敏 |
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| 作者单位： | 重庆烟草工业有限责任公司技术部;川渝中烟工业有限责任公司技术中心 |
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| 摘 要： | 为提高烟草中蛋白质的分析速度,对流动分析法中蛋白质的前处理过程进行了改进,即用萃取除可溶性氮代替加热.并对检测结果进行了比较,结果表明:用萃取代替加热在批量检测时可大幅减少操作强度,蛋白质的回收率为94.9%～102.9%,变异系数小于2.77%;检测结果与文献方法无显著性差异,可用于批量烟草样品中蛋白质含量的快速分析.
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| 关 键 词： | 流动分析法 烟草 蛋白质 前处理 |
Improvement on preparation for protein determining in tobacco with enzymolysis-autoanalyzer |
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| LI Qiu-tong,WANG Peng,SHANG Jun,LV Xiang-min.Improvement on preparation for protein determining in tobacco with enzymolysis-autoanalyzer[J].Journal of Hunan University of Arts and Science:Natural Science Edition,2012(3):57-59,76. |
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| Authors: | LI Qiu-tong WANG Peng SHANG Jun LV Xiang-min |
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| Institution: | 1(1.Chongqing Tobacco Industrial Co.,Ltd.,Chongqing 400060,China;2.China Tobacco Chuanyu Industrial Corporation,Chengdu 610066,China) |
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| Abstract: | The preparation for enzymolysis-Autoanalyzer of protein in tobacco was improved to increase the analytic rate,extraction instead of heating.The results from extraction separation and heating were compared.The results indicated that,instead of heating using extraction separation can be a substantial increase in volume of tobacco sample pretreatment rate,the average recovery of starch was 94.9%~102.9%,RSD < 2.77%.And the test results with the original method was no significant difference,It could be applied in bulk tobacco samples rapid analysis of protein in tobacco. |
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| Keywords: | enzymolysis-autoanalyzer tobacco protein preparation |
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