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半胱胺对鸡十二指肠中肥大细胞数量的影响
引用本文:练高建,杨倩.半胱胺对鸡十二指肠中肥大细胞数量的影响[J].湖南文理学院学报(自然科学版),2012(3):33-35,45.
作者姓名:练高建  杨倩
作者单位:南京农业大学动物医学院;南华大学实验动物学部
基金项目:国家自然科学基金项目(30070564)
摘    要:84只1日龄小公鸡,分为四组,分别为对照组、新城疫弱毒疫苗免疫组(ND组)、饲喂半胱胺的试验组(CS组)以及饲喂半胱胺后,再用新城疫弱毒疫苗进行消化道粘膜的试验组(CN组).应用甲苯胺蓝染色技术研究了十二指肠中肥大细胞数量的变化.结果显示:新城疫疫苗首免后第三周,CN组十二指肠中肥大细胞数量比ND组明显增加(P0.01);首免后第五周,各试验组十二指肠中肥大细胞的数量均比对照组多,尤其是CN组,可能因为个体差异较大,各组间并无显著差异.本试验的结果提示,肥大细胞的数量可能反映了消化道粘膜的免疫水平.

关 键 词:半胱胺    十二指  肥大细胞

Study of the effect of cysteamine on the change of mast cells number in chicken duodenum
LIAN Gao-jian,YANG Qian.Study of the effect of cysteamine on the change of mast cells number in chicken duodenum[J].Journal of Hunan University of Arts and Science:Natural Science Edition,2012(3):33-35,45.
Authors:LIAN Gao-jian  YANG Qian
Institution:1(1.College of Veterinary Medicine,Nanjing Agric Univ,Nanjing 210095,China;2.Department of Laboratory Animal Science,Univ of South China,Hengyang 421001,China)
Abstract:Eight-four 1-day-old cock were divided into control group,ND treated group,CS treated group and the group was immunized with Newcastle disease attenuated vaccine by digestive rout after feeding cysteamine(CN group).Toluidine blue staining method was used to study the change of mast cells number in chicken small intestinal.The results showed that the number of mast cells in duodenum of CN group increased significantly(P<0.01) compared to the group immunizing only with Newcastle disease attenuated vaccine at 3 week after oral immunization;Comparing to the control group at 5 week,the number of mast cells among experiment group increased significantly,though no differences existed among all groups.Our results suggest the number of mast cells might reflect the digestive mucosal immunization.
Keywords:cysteamine  chicken  duodenum  mast cells
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