| 玫瑰冠鸡IGF-1基因多态性与早期生长速度关系研究 |
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| 引用本文： | 李伟,李大全,李应生,孙凤霞,彭夏雨.玫瑰冠鸡IGF-1基因多态性与早期生长速度关系研究[J].石河子大学学报,2007,25(3):319-322. |
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| 作者姓名： | 李伟 李大全 李应生 孙凤霞 彭夏雨 |
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| 作者单位： | 石河子大学动物科技学院 新疆石河子832003 |
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| 摘 要： | 以玫瑰冠类群鸡(玫瑰冠鸡)为材料,采用PCR-RFLP法检测了IGF-1基因5’调控区DNA序列多态性,并运用线性模型统计方法分析了多态性与初生重和12周龄体重的关系。结果显示:玫瑰冠鸡IGF-1基因5’调控区自然存在两种不同DNA序列,经PstI酶切后出现3种基因型("-/-"、"-/ "、" / "),基因型分布符合哈代-温伯格定律。各基因型个体的初生重、12周龄体重的最小二乘均存在"-/-">" /-">" / "的趋势,且"-/-"型个体的12周龄体重显著高于" / "型个体(P<0.05)。
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| 关 键 词： | 玫瑰冠鸡 IGF-1基因 PCR-RFLP DNA多态性 体重 |
| 文章编号： | 1007-7383(2007)03-0319-04 |
| 修稿时间： | 2007年4月10日 |
Correlation Analysis between Polymorphism of the IGF-1 Gene and Body Growth in Rose Cockscomb Chickens |
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| LI Wei,LI Da-quan,LI Ying-sheng,SUN Feng-xia,PENG Xia-yu.Correlation Analysis between Polymorphism of the IGF-1 Gene and Body Growth in Rose Cockscomb Chickens[J].Journal of Shihezi University(Natural Science),2007,25(3):319-322. |
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| Authors: | LI Wei LI Da-quan LI Ying-sheng SUN Feng-xia PENG Xia-yu |
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| Abstract: | The genetic variation of the 5'region of the IGF-I gene in rose cockscomb chickens was detected by PCR-RFLP.The relationship between DNA polymorphisms revealed by this method and bodyweight were ana1yzed with linear model.The results showed that there were three genotypes("-/-","-/ "and" / ")after PCR products were cut by Pst I enzyme and the observed distribution of genotype was not different from the expected distribution under the assumption of the Hardy-Weinberg equilibrium for the IGF-I allele.There was a tendency that the genotype class Pst I(-/-)had the least square means of body weight for 12 weeks and birth in three genotype classes,while the genotype class Pst I(-/ )was intermediate between Pst I(-/-)and Pst I( / )class.Furthermore,Pst I(-/-)genotypes was associated with a significantly higher body weight for 12 weeks than the Pst I( / ) genotypes(P<0.05). |
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| Keywords: | PCR-RFLP |
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