| 石油降解菌的筛选及其降解能力的初步研究 |
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| 引用本文： | 杨乐,李春,鲁建江,王开勇.石油降解菌的筛选及其降解能力的初步研究[J].石河子大学学报,2007,25(4):484-487. |
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| 作者姓名： | 杨乐 李春 鲁建江 王开勇 |
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| 作者单位： | [1]石河子大学绿色合成与转化重点实验室,新疆石河子832003 [2]石河子大学农学院,新疆石河子832003 [3]石河子大学生命科学学院,新疆石河子832003 [4]北京理工大学生命科学与技术学院,北京100081 |
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| 基金项目： | 新世纪优秀人才支持计划 |
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| 摘 要： | 为了获得高效石油降解菌株，以原油为唯一碳源，从克拉玛依石油污染土壤中分离筛选得到14株细菌，利用紫外分光光度法、氯化三苯基四氮唑法和吐温80法对其石油降解能力进行了研究。结果表明：菌株中M3、M7、M9和M11的石油降解能力较高，降解率分别达到71．6％、56．2％、88．2％和60．3％，且这些菌株均有较高的TTC-脱氢酶活性和脂酶活性，因此可以根据两种酶活性的高低初步判断菌株的石油降解能力。
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| 关 键 词： | 石油降解菌 降解率 TTC-脱氢酶 脂酶 |
| 文章编号： | 1007-7383（2007）04-0484-04 |
| 修稿时间： | 2007-04-19 |
Preliminary Study on Degradation and Isolation of Bacteria for Degradation of Petroleum Hydrocarbon |
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| YANG Le,LI Chun,LU Jian-jiang,WANG Kai-yong.Preliminary Study on Degradation and Isolation of Bacteria for Degradation of Petroleum Hydrocarbon[J].Journal of Shihezi University(Natural Science),2007,25(4):484-487. |
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| Authors: | YANG Le LI Chun LU Jian-jiang WANG Kai-yong |
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| Institution: | 1 Key Laboratory of Green Chemical Technology, Shihezi University, Shihezi, Xinjiang 832003, China; 2 College of Agriculture, Shihezi University, Shihezi, Xinjiarlg 832003, China; 3 College of Life Science, Shihezi University, Shihezi Xinjiang 832003, China; 4 School of Life Science and Technology, Beijing University of Technology, Beijing 100081, China |
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| Abstract: | In order to screen high-effective oil-degrading strains, fourteen strains of bacteria sole carbon source from oil contaminated soil of Kelamayi. Their abilities of oil degradation spectrophotometry, TFC method andTween80 method. The results showed that the degradat were isolated with crude oil as were investigated by ultraviolet ion ability of Strains M3, M7, M9 and M11 were better than others. The oil degradation rates of d strains were 71.6%, 56.2%, 88.2% and 60.3% respectively, and they had TFC dehydrogenase activity and esterase activity. TTC-dehydrogenase activity and esterase activity can be used to judge microbe ability for oil degradation. |
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| Keywords: | oil degradation bacteria degradation rates TTC-dehydrogenase esterase |
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