| 石油降解菌的筛选及其产表面活性剂的研究 |
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| 引用本文： | 杨乐,鲁建江,王开勇,庞玮,李春.石油降解菌的筛选及其产表面活性剂的研究[J].石河子大学学报,2009,27(2). |
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| 作者姓名： | 杨乐 鲁建江 王开勇 庞玮 李春 |
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| 作者单位： | 杨乐,王开勇,庞玮,YANG Le,WANG Kaiyong,PANG Wei(石河子大学农学院,石河子,832003);鲁建江,LU Jian Jiang(石河子大学绿色合成与转化重点实验室,石河子,832003);李春,LI Chun(石河子大学绿色合成与转化重点实验室,石河子,832003;北京理工大学生命科学与技术学院,北京,100081)
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| 摘 要： | 从克拉玛依油田土壤分离纯化出3株石油降解菌,经鉴定DM-1和DM-3为芽孢杆菌属细菌,DM-2为假单孢菌属细菌,菌株DM-1,DM-2和DM-3降解率分别达到59.02%,62.02%和73.51%.排油实验、表面张力和油水乳化稳定性测定表明3株菌产生的表面活性剂能降低液体表面张力,具有较强的乳化原油的能力.实验证明菌株DM-1,DM-2和DM-3产脂肽、脂蛋白类表面活性物质,表面活性剂的产生为生长相关型,在对数生长期产表面活性剂,48小时浓度达到最大,分别为1.78g/L,2.15g/L和2.75g/L.
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| 关 键 词： | 石油降解菌 降解率 生物表面活性剂 |
Isolation of Bacteria for Degradation of Petroleum Hydrocarbon and Its Characteristics of Biosurfactants |
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| YANG Le,LU Jian Jiang,WANG Kaiyong,PANG Wei,LI Chun.Isolation of Bacteria for Degradation of Petroleum Hydrocarbon and Its Characteristics of Biosurfactants[J].Journal of Shihezi University(Natural Science),2009,27(2). |
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| Authors: | YANG Le LU Jian Jiang WANG Kaiyong PANG Wei LI Chun |
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| Institution: | 1 College of Agriculture;Shihezi University;Shihezi 832003;China;2 Laboratory for Green Chemical Technology;3 School of Life Science and Technology;Beijing Institute of Technology;Beijing 100081;China |
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| Abstract: | Three strains of bacteria were isolated with crude oil as sole carbon source from oil contaminated soil of Kelamayi.Strains DM-1 and DM-3 both belonged to Bacillus sp.and strains DM-2 belonged to Pseudomonaa sp.identified by momhological and biological tests.The oil degradation rates of three strains were 59.02%,62.02% and 73.51% respectively.The experiments of oil displacement,surface tension and the emulsion stability showed that biosurfactant produced by three bacterial strains could reduce the surface t... |
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| Keywords: | oil degradation bacteria degradation rates biosurfactant |
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