| 新疆绵羊种布鲁氏菌GroEL基因的克隆及序列分析 |
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| 引用本文： | 刘辉,陈创夫,许程剑,王远志,韩亚杰.新疆绵羊种布鲁氏菌GroEL基因的克隆及序列分析[J].石河子大学学报,2005,23(6):683-686. |
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| 作者姓名： | 刘辉 陈创夫 许程剑 王远志 韩亚杰 |
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| 作者单位： | 新疆地方与民族高发病实验室,新疆石河子832003 |
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| 摘 要： | 参考牛种布鲁氏菌的GroEL（热休克蛋白）基因设计引物，扩增出新疆绵羊种布鲁氏菌GroEL基因片段，将其片段克隆到T载体上测序。结果表明：新疆源布鲁氏菌GroEL基因片段长1641bp，编码546个氨基酸，与羊种（B．melitensis）、猪种（B．suis）以及牛种（B．abortus）GroEL基因的核苷酸序列同源性分别为99．88％、99．82％、99．88％，推导的氨基酸序列同源性在99％以上，显示了很强的保守性。
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| 关 键 词： | 布鲁氏菌 GroEL基因 克隆 序列分析 |
| 文章编号： | 1007-7383(2005)06-0683-04 |
| 收稿时间： | 2005-11-09 |
| 修稿时间： | 2005年11月9日 |
Cloning and Sequence Analysis of GroEL Gene of Brucella ovis Strain 80/019 Isolated from Xinjiang |
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| LIU Hui, CHEN Chuang-fu, XU Chen-jian, WANG Yuan-zhi, HAN Ya-jie.Cloning and Sequence Analysis of GroEL Gene of Brucella ovis Strain 80/019 Isolated from Xinjiang[J].Journal of Shihezi University(Natural Science),2005,23(6):683-686. |
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| Authors: | LIU Hui CHEN Chuang-fu XU Chen-jian WANG Yuan-zhi HAN Ya-jie |
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| Abstract: | A pair of primers was designed and synthesized based on the nucleotide sequence of GroEL gene of brucella abortus. One target fragment about 1.6kb was amplified via PCR from the genomie DNA of Brucella ovis strain 80/019 isolated from Xinjiang with the designed specific primers. After purification, the target fragment was cloned into linearized plasmid vector PBS-T and sequenced. The ORF of GroEL gene was 1641bp, and the nueleotide sequence homology of GroEL gene of brucella ovis with that of brueella abrotus, brueella melitensis, brueella suis, was 99.88%, 99.88%, 99.82% respectively,and a high degree of amino acid sequence homology was revealed so the nucleotide sequence and amino acid of homology is the highest. |
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| Keywords: | brucella GroEL gene clone sequence analysis |
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