啤酒花潜隐病毒新疆分离物的全基因组序列分析

对新疆啤酒花上获得的HpLV分离物HpLV-XJ进行了全长克隆和基因组序列分析。结果显示:HpLV-XJ的全基因组序列为8612个核苷酸(nt)(不包括poly A),含有6个开放阅读框(ORF),分别编码224 kDa(ORF1)、25kDa(ORF2)、11 kDa(ORF3)、7 kDa(ORF4)、34 kDa(ORF5)、和12 kDa(ORF6)蛋白。序列相似性分析结果表明,HpLV-XJ与HpLV(GenBank:AB032469)序列相似性达98.5%,6个开放阅读框的核苷酸序列相似性分别为98.3%、99.0%、97.6%、96.7%、99.5%和98.4%;由此推导的氨基酸序列相似性分别为98.6%、98.7%、97.2%、95.0%、99.4%和98.1%,各个基因的核苷酸序列和蛋白的氨基酸之间存在着一定的差异。

The Complete Nucleotide Sequence of the RNA of Hop Latent Virus Xinjiang Isolate

The isolate HpLV-XJ was obtained from hop in Xinjiang.Its complete nucleotide sequence of the genomic RNA was determined by RT-PCR and sequencing.The results show that HpLV-XJ consists of 8612 nucleotides(nt).excluding the 3 terminal poly(A) tail,and contains six open reading frames(ORFs),which encode putative proteins of 224 kDa(ORF1),25 kDa (ORF2),11 kDa(ORF3),7 kDa(ORF4),34 kDa(ORF5) and 12 kDa(ORF6).The sequence of nucleotide identities between HpLV-XJ and HpLV strains are 98.47 percent.The nucleotide sequence of six ORFs identities between HpLV-XJ and HpLV are 98.3%,99.0%,97.6%,96.7%,99.5%and 98.4%,respectively.The identity of amino sequence deduced by six ORFs between HpLV-XJ and HpLV are 98.64%,98.7%,97.2%,95.0%,99.4%and 98.1%,respectively.There are some mutation of the nucleotide and amino acid.