| 利用mRNA差异显示技术筛选绵羊毛性状相关基因 |
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| 引用本文： | 牟艳军,刘国龄,廖和荣,沙莎,赵宗胜,李大全.利用mRNA差异显示技术筛选绵羊毛性状相关基因[J].石河子大学学报,2006,24(1):95-97. |
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| 作者姓名： | 牟艳军 刘国龄 廖和荣 沙莎 赵宗胜 李大全 |
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| 作者单位： | [1]石河子大学动物科技学院,新疆石河子832003 [2]新疆自治区畜牧厅畜禽改良总站,新疆乌鲁木齐830009 |
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| 摘 要： | 利用mRNA差异显示技术（DD-PCR）,比较绵羊肩、腹、腿三部位皮肤中mRNA的差异表达,共获得差异表达的cDNA片段16条,其中肩部、腹部与腿部之间的差异较大;对所有16条差异片段进行测序并在GENBANK中进行检索,未发现有同源序列.提示16条cDNA片段可能为未发现的基因片段,也可能存在假阳性片段.
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| 关 键 词： | 绵羊 皮肤 羊毛性状 mRNA差异显示技术 |
| 文章编号： | 1007-7383（2006）01-0095-03 |
| 收稿时间： | 2005-10-21 |
| 修稿时间： | 2005年10月21 |
Isolation of Genes Associated with Wool Traits by mRNA Differential Display |
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| MU Yan-jun,LIU Guo-ling,LIAO He-rong,SHA Sha,ZHAO Zong-sheng,LI Da-quan.Isolation of Genes Associated with Wool Traits by mRNA Differential Display[J].Journal of Shihezi University(Natural Science),2006,24(1):95-97. |
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| Authors: | MU Yan-jun LIU Guo-ling LIAO He-rong SHA Sha ZHAO Zong-sheng LI Da-quan |
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| Abstract: | mRNA expression in different parts of sheep skin(shoulder,abdomen and midleg)were compared by mRNA differential display technique.16 differential cDNA fragments were obtained.Among them,differences between shoulder and mid-leg were maximum.Then their sequences were determined and blasted in GENBANK,but homologous sequences were not detected.So 16 cDNA fragments may be sequences,but had been not found or some of them might be false positive fragments.The results lay basisfor studies on genes associated with wool traits and functions of these genes. |
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| Keywords: | sheep skin wool traits mRNA differential display |
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