| 绵羊肺炎支原体PCR检测方法的建立 |
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| 引用本文： | 屈勇刚,剡根强,陈宏伟,杨志军,张再清,李刚.绵羊肺炎支原体PCR检测方法的建立[J].石河子大学学报,2005,23(6):687-689. |
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| 作者姓名： | 屈勇刚 剡根强 陈宏伟 杨志军 张再清 李刚 |
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| 作者单位： | [1]石河子大学动物科技学院,新疆石河子832003 [2]军事医学科学院军事兽医研究所,吉林长春130062 [3]塔里木大学动物科技学院,新疆阿拉尔843000 [4]新疆生产建设兵团农八师150团兽医站,新疆石河子832055 |
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| 基金项目： | 国家自然科学基金（20376041）;国家重点基础研究发展规划项目（2003CB716004） |
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| 摘 要： | 为检测绵羊肺炎支原体感染，根据公开发表的绵羊肺炎支原体16SrRNA序列设计特异性引物。建立了PCR诊断方法。通过对绵羊肺炎支原体标准株、临床分离株、丝状支原体山羊亚种及临床病羊鼻拭子的检测。发现应用该检测方法能够对标准株、分离株和临床病羊鼻拭子扩增出特异性产物，而对丝状支原体山羊亚种则扩增小出。证实所建方法在绵羊支原体性肺炎的诊断上具有特异性、相对快速、简便等优点，值得在临床中推广应用。
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| 关 键 词： | 绵羊肺炎支原体 PCR 16SrRNA |
| 文章编号： | 1007-7383(2005)06-0687-03 |
| 收稿时间： | 2005-11-01 |
| 修稿时间： | 2005年11月1日 |
Establishment of PCR Method to Examine Mycoplasma ovipneumoniae |
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| QU Yong-gang, YAN Gen-qiang, CHEN Hong-wei, YANG Zhi-jun, ZHANG Zai-qing, LI Gang.Establishment of PCR Method to Examine Mycoplasma ovipneumoniae[J].Journal of Shihezi University(Natural Science),2005,23(6):687-689. |
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| Authors: | QU Yong-gang YAN Gen-qiang CHEN Hong-wei YANG Zhi-jun ZHANG Zai-qing LI Gang |
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| Institution: | 1. College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, China; 2. Institute of Military Veterinary, Academy of Military Medical Sciences, Changehun, Jilin 130062, China; 3. College of Animal Science and Technology, Tarim university, Mar, Xinjiang 843000, China; 4.Veterinary Station of the 150th State Farm, 8th Agricultural Division, Shihezi, Xinjiang 832055, China |
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| Abstract: | To examine the infection of Mycoplasma ovipneumoniae,two specific primers was designed according to the published 16SrRNA sequence of Mycoplasma ovipneumoniae,then a PCR detection method was established.After examining some samples by the PCR method,including standard strain of Mycoplasma ovipneumoniae Y98,three isolated strains of Mycoplasma ovipneumoniae,one strain of Mycoplasma mycoides subsp Capri and secretion of ill sheep,the specific product could be amplified from Y98,three isolated strains and secretion of ill sheep.But the strain of Mycoplasma mycoides subsp Capri did not.The result indicated that the method that we constructed is specific,relatively fast and simple.It's worth applying in clinic. |
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| Keywords: | Mycoplasma ovipneumoniae PCR 16SrRNA |
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