| 家鸡单核细胞趋化因子MCP-1克隆、融合表达及序列分析 |
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| 引用本文： | 马磊,张婷婷,杨微,吕鲁杰.家鸡单核细胞趋化因子MCP-1克隆、融合表达及序列分析[J].石河子大学学报,2008,26(6). |
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| 作者姓名： | 马磊 张婷婷 杨微 吕鲁杰 |
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| 作者单位： | 石河子大学生命科学学学院，西北农林科技大学生命科学学院，西北农林科技大学林学院 |
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| 摘 要： | 本研究从鸡cDNA文库中,通过菌落PCR筛选获得单核细胞趋化激活因子(MCP-1)基因的cDNA,将该cDNA中的ORF插入到pET-28a( )质粒(携带T7/lac启动子序列和His-Tag标签序列)中,得到pET-mcp-1质粒;重组质粒转化E.coliBL21(DE3)后,经IPTG诱导表达得到MCP-1和His-Tag的融合蛋白(HisTag-MCP-1)。该基因不仅具有CC趋化因子家族Cys-Cys氨基酸模序,并且与结构和功能相关的氨基酸高度保守。
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| 关 键 词： | MCP-1 Cys-Cys氨基酸模序 CC趋化因子家族 鸡 |
Cloning,Expression and Sequence Analysis of Chicken Monocyte Chemoattractant Protein-1 |
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| MA Lei,ZHANG Ting-ting,YANG Wei,L Lu-jie.Cloning,Expression and Sequence Analysis of Chicken Monocyte Chemoattractant Protein-1[J].Journal of Shihezi University(Natural Science),2008,26(6). |
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| Authors: | MA Lei ZHANG Ting-ting YANG Wei L Lu-jie |
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| Institution: | MA Lei,ZHANG Ting-ting,YANG Wei,L(U) Lu-jie |
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| Abstract: | A cDNA,chicken monocyte chemoattractant protein-1(MCP-1) gene,was cloned by PCR-screening in a cDNA library.The mcp-1 ORF was inserted into the expression vector pET-28a( ),a T7/lac promoter-based and His-Tag-based fusion plasmid.Then the recombinant plasmid(pET-mcp-1) was transformed into E.coli BL21(DE3).The fusion protein of His-Tag and MCP-1(His-Tag-MCP-1) was expressed by induction of IPTG.The chicken MCP-1 amino acid residual sequence contained the conserved Cys-Cys motif characterized as the CC chemokines family,and was significantly conserved at amino acid residuals involved in structure and function. |
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| Keywords: | MCP-1 CC chemokines family Cys-Cys motif chicken |
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