| 新疆棉花枯萎病菌7号生理小种的AFLP分析 |
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| 引用本文： | 张莉,陈文霞,刘桂伶,崔燕华.新疆棉花枯萎病菌7号生理小种的AFLP分析[J].石河子大学学报,2009,27(3):298-301. |
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| 作者姓名： | 张莉 陈文霞 刘桂伶 崔燕华 |
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| 作者单位： | 张莉,刘桂伶,崔燕华,ZHANG Li,LIU Guiling,CUI Yanhua(石河子大学绿洲农作物病害防控重点实验室,石河子,832003);陈文霞,CHEN Wenxia(新疆兵团农四师农科所,伊宁,835004)
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| 基金项目： | 教育部春晖计划科研合作项目 |
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| 摘 要： | 采用AFLP技术对新疆棉花枯萎病菌7号生理小种的36菌株和2个标准枯萎病菌菌株进行了DNA指纹分析。结果表明：11对AFLP引物对供试菌株扩增出287条带,其中多态性带99条,占总带数的34.49%,棉花枯萎病菌7号小种的遗传距离变化在0.41~0.95间,平均为0.71,表明新疆棉花枯萎病菌的遗传多样性较高。利用NTSYS pc软件中UPGMA算法构建了新疆棉花枯萎病菌的亲缘关系树状图,聚类分析结果表明：供试菌系的AFLP指纹多态性与致病力多态性不相关,但枯萎病菌遗传多样性与病原菌的地理来源有一定的相关性。
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| 关 键 词： | 棉花枯萎病菌 遗传多样性 AFLP |
Genetic Diversity of Xinjiang Isolates of Fusarium oxysporum f.sp.asinfectum Revealed by AFLP Analysis |
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| ZHANG Li,CHEN Wenxia,LIU Guiling,CUI Yanhua.Genetic Diversity of Xinjiang Isolates of Fusarium oxysporum f.sp.asinfectum Revealed by AFLP Analysis[J].Journal of Shihezi University(Natural Science),2009,27(3):298-301. |
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| Authors: | ZHANG Li CHEN Wenxia LIU Guiling CUI Yanhua |
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| Institution: | 1 Key Laboratory of Prevention and Control for Oasis Crop Disease;Shihezi University;Shihezi 832003;China;2 Institute of Agricultural Sciences;Agricultural Division 4;Yi'ning;Shihezi 835004;China |
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| Abstract: | 36 isolates from Xinjiang and 2 standard isolates of Fusarium oxysporum f.sp.Vasinfectum were analyzed genetic diversity by using AFLP technology.The results showed:11 combinations of primer pair were used to amplify the genomic DNA of isolates.A total of 287 AFLP bands were obtained,and 99 of them,covering 34.49%,showed polymorphism.Genetic distance of 38 isolates based on AFLP markers ranged from 0.41 to 0.95,averaged 0.71.This showed that the genetic diversity was high among the tested isolates.When all ... |
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| Keywords: | AFLP |
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