| 大鼠脑损伤海马神经干细胞bFGF表达及三七总皂苷的作用研究 |
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| 引用本文： | 张建平,孙弋,司银楚.大鼠脑损伤海马神经干细胞bFGF表达及三七总皂苷的作用研究[J].井冈山学院学报,2008,29(2):72-77. |
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| 作者姓名： | 张建平 孙弋 司银楚 |
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| 作者单位： | 井冈山大学医学院 江西吉安343009(张建平，孙弋)，北京中医药大学解剖学教研室 北京100029(司银楚) |
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| 基金项目： | 教育部重点研究课题(104036) |
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| 摘 要： | 目的探讨脑损伤对神经干细胞bFGF阳性细胞表达的影响及三七总皂苷的作用。方法通过免疫细胞化学的方法检测脑损伤后以及给予三七总皂苷后新生大鼠海马神经干细胞bFGF阳性细胞的表达。结果三七总皂苷可促进缺血再灌注组的bFGF阳性细胞的数目明显增多。缺血组1h、2h的bFGF阳细胞计数均多于正常组；模型组6h后阳性细胞计数开始低于正常组，具有统计学意义。24h给药组的bFGF阳性细胞的面密度和光密度均高于模型组，有显著性差异伙0.001），具有统计学意义。结论体外模拟脑缺血在一定时间内能引起海马神经干细胞内的bFGF水平上调，三七总皂苷对bFGF水平的上调具有促进作用。
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| 关 键 词： | 脑缺血再灌注 海马神经干细胞 碱性成纤维生长因子 三七总皂苷 |
| 文章编号： | 1673-4718（2008）02-0072-04 |
| 收稿时间： | 2007-11-02 |
| 修稿时间： | 2007年11月2日 |
bFGF expression of hippocampus neural stem cells in rat brain injured and investigation of panax pseudo-ginseng effect |
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| ZHANG Jian-ping,SUN Yi,SI Yin-chu.bFGF expression of hippocampus neural stem cells in rat brain injured and investigation of panax pseudo-ginseng effect[J].Journal of Jinggangshan University,2008,29(2):72-77. |
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| Authors: | ZHANG Jian-ping SUN Yi SI Yin-chu |
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| Institution: | ZHANG Jian-ping, SUN Yi, SI Yin-chu(1.Medical School,Jinggangshan University, Jian 343009 China; 2.Department of Anatomy,Beijing University of Traditional Chinese Medicine,Beijing 100029, China) |
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| Abstract: | Objective: To investigate the influences of brain injured on the expression of bFGF positive neural stem cells and effect of panax pseudo-ginseng;Methods: To detect the expression of bFGF positive cells of neonate rat hippocampus neural stem cells after the operation of brain injured and the treatment of Panax Notoginseng saponins by the immunocytochemistry methods.Results: Panax notoginseng saponins can significantly increase the number of bFGF positive neurons of RP groups.Compared with normal group,the count number of the bFGF positive cells in 1h and 2h cerebral group is much greater.and bFGF positive cells begins to be lower than normal group after 6h and with statistical significance;Compared with model group,the area density and optical density of bFGF positive neurons in the 24h treatment group are all greater.Conclusion: Cerebral ischemia can up-regulate the bFGF level in the hippocampus neural stem cells with a period of time,and panax notoginseng saponins promotes up-regulation of the bFGF level. |
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| Keywords: | cerebral ischemia and reperfusion hippocampus neural stem cell bFGF panax notoginseng saponins |
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