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多元校正荧光法同时测定α-萘乙酸和吲哚-3-乙酸
引用本文:黄春芳,陈华龙,龙文清.多元校正荧光法同时测定α-萘乙酸和吲哚-3-乙酸[J].井冈山学院学报,2008,29(6):52-53.
作者姓名:黄春芳  陈华龙  龙文清
作者单位:井冈山大学化学化工学院 江西省配位化学重点实验室,井冈山大学化学化工学院,江西省配位化学重点实验室,井冈山大学化学化工学院,江西省配位化学重点实验室,江西吉安343009,江西吉安343009,江西吉安343009
基金项目:吉安市重点科技项目  
摘    要:在紫外光激发下,植物生长调节剂萘乙酸(NAA)和吲哚-3-乙酸(IAA)均能发射荧光,最大激发/发射波长分别为λex/em=291/337nm,λex/em=287/371nm。研究了酸度、光照时间、温度及共存物对荧光强度的影响。建立了多元校正不经分离同时测定NAA和IAA两组分的新方法。NAA和IAA线性范围分别是6.44×10^-8-29×10^-6mol·L^-1,1.94×10^-8-3.88×10^-7mol·L^-1;最低检测限为2.10×10^-8mol·L^-1,1.08×10^-8mol·L^-1。该法对两组分合成样及强化水样均有较好的解析能力,回收率在92%-103%之间。

关 键 词:荧光  α-萘乙酸  吲哚-3-乙酸  多元校正

Application of multivariate calibration to simultaneous fluorescence determination of α-naphthylaceticacid and indole-3-aceticacid
HUANG Chun-fang,CHEN Hua-long,LONG Wen-qing.Application of multivariate calibration to simultaneous fluorescence determination of α-naphthylaceticacid and indole-3-aceticacid[J].Journal of Jinggangshan University,2008,29(6):52-53.
Authors:HUANG Chun-fang  CHEN Hua-long  LONG Wen-qing
Institution:(School of Chemistry and Chemical Engineering, Jinggangshan University, Jiangxi Province Key Laboratory of Coordination Chemistry, Ji'an, 343009, China)
Abstract:A method has been developed for simultaneous determination of α-Naphthylacetic acid(NAA) and Indole-3-acetic acid (IAA) in water samples. It relies on the fluorescence spectra of NAA and IAA with the aid of multivariate calibration such as multiple linear regression (MLR), principal component regression (PCR) and partial least squares regression(PLS). The experimental conditions were studied in details. Linear calibration graphs were obtained in the concentration range of 6.44×10^-8-1.29×10^-6mol·L^-1 and 1.94×10^-8- 3.88×10^-7 mol·L^-1 for NAA and IAA, respectively. The detection limit of NAA was 2.10×10^-8 mol·L^-1, while 1.08×10^-8 mol·L^-1 for IAA. Synthetic binary mixtures were analyzed, and the data obtained were processed by multivariate calibration. The method was also applied for determination of NAA and IAA in several spiked water samples with satisfactory recoveries.
Keywords:fluorescence  α-naphthyl-aeetieaeid  indole-3-aceticacid  multivariate calibration
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