| 大麻脱胶高酶活菌株的诱变选育 |
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| 引用本文： | 程海,刘自殚,冯瑞良,吕忠.大麻脱胶高酶活菌株的诱变选育[J].东华大学学报(自然科学版),2001,27(2):91-95. |
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| 作者姓名： | 程海 刘自殚 冯瑞良 吕忠 |
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| 作者单位： | 山东大学微生物技术国家重点实验室济南 250100 |
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| 基金项目： | 国家科委生物技术中心资助项目 |
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| 摘 要： | 原始菌株Bacillus sp.No.46经紫外诱变后，挑取76株进行摇瓶初，复筛，复得10株诱变株，果胶酶酶活高于原菌株20％－30％，聚半乳糖醛酸酸酶活提高35％－70%，连续液大麻脱胶试验残胶率低于10％，脱胶后大麻纤维特数可达19－11，脱胶后酶活酶活损失约20％，脱胶酶液至少可用2次以上。
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| 关 键 词： | U.V诱变 果胶酶 木聚糖酶 大麻 脱胶 高酯活菌株 诱变选育 |
| 修稿时间： | 2000年2月14日 |
U.V. Inducing and Screening Tests of High Enzyme Activity Strains in Degumming |
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| Cheng hai,Liu zi-rong,Feng rui-liang,La zhong.U.V. Inducing and Screening Tests of High Enzyme Activity Strains in Degumming[J].Journal of Donghua University,2001,27(2):91-95. |
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| Authors: | Cheng hai Liu zi-rong Feng rui-liang La zhong |
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| Abstract: | Seventy-six strains were induced from the original strain (Bacillus sp. No.46)by the ultraviolet light. Ten induced strains were obtained after primary screen tests and retests. The pectinase activity of ten induced strains was about 20% ~ 30% higher than that of the original one. The xylanase activity increased about 35%~70% . The characteristic in producing the pectinase remained unchanged after five generations. The degumming tests of the hemp showed there was less than 10% gum left in the hemp after fermentation. The fibre number of the hemp after fermentation was 500 ~ 850. The loss of the enzyme' s activity in degumming was about 20 % . So the degumming solution could be used at least twice. |
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| Keywords: | U V inducing pectinase polygalacturonase |
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