| 对甲氧基苯甲醛为荧光探针测定DNA含量 |
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| 引用本文： | 宛瑜,叶玲,吴翚.对甲氧基苯甲醛为荧光探针测定DNA含量[J].淮阴师范学院学报(自然科学版),2007,6(4):316-319. |
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| 作者姓名： | 宛瑜 叶玲 吴翚 |
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| 作者单位： | 1. 徐州师范大学,化学化工院,江苏,徐州,221116;江苏省药用植物生物技术重点实验室,江苏,徐州,221116
2. 徐州师范大学,化学化工院,江苏,徐州,221116 |
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| 基金项目： | 国家自然科学基金资助项目(20772103)，江苏省自然科学基金资助项目(BK2007028) |
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| 摘 要： | 基于DNA与对甲氧基苯甲醛的荧光猝灭效应,以甲氧基苯甲醛作为荧光探针建立了一种简便快速测定DNA的荧光分析方法.考察了pH、荧光探针浓度、温度、放置时间等条件对荧光探针及DNA测定的影响.该方法测定DNA含量的线性范围为51~1 000 ng/mL,检出限为30 ng/mL,10次测量500 ng/mL DNA的相对标准偏差为2.0%,可用于合成样品的测定.
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| 关 键 词： | 对甲氧基苯甲醛 荧光探针 鲱鱼精DNA |
| 文章编号： | 1671-6876（2007）04-0316-04 |
| 收稿时间： | 2007-10-08 |
| 修稿时间： | 2007年10月8日 |
Determination of Nucleic Acids Using p-methoxybenzaldhyde as Fluorescence Probe |
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| WAN Yu,YE Ling,WU Hui.Determination of Nucleic Acids Using p-methoxybenzaldhyde as Fluorescence Probe[J].Journal of Huaiyin Teachers College(Natrual Science Edition),2007,6(4):316-319. |
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| Authors: | WAN Yu YE Ling WU Hui |
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| Institution: | 1. College of Chemistry and Chemical Engineering, Xuzhou Normal University, Xuzhou Jiangsu 221116, China;2. KeyLaboratory of Biotechnology on Medical Plant of Jiangsu Province, Xuzhou Jiangsu 221116, China |
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| Abstract: | A symmetric emission spectrum and a broad,continous excitation spectrum was given by p-methoxybenzaldhyde.A rapid determination of DNA has been developed based on its fluorescence quenching ability on the endogenous fluorescence of p-methoxybenzaldhyde(I).The influence of pH,concentration of I,temperature and placing time on the determination were studied in detail.Under optimum conditions,the calibration curve is linear over the range 51.0～1000.0 ng/mL,with a detection limit of 30.0 ng/mL.The relative standard deviation of 500.0 ng/mL DNA is 2.0%.This method is simple,rapid and sensitive and shows promise in the application of synthetic samples. |
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| Keywords: | p-methoxybenzaldhyde DNA fluorescence probe |
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