| 固定化桔青霉发酵核酸酶P1的研究 |
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| 引用本文： | 王克明,王宇光.固定化桔青霉发酵核酸酶P1的研究[J].浙江科技学院学报,2003,15(2):90-93. |
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| 作者姓名： | 王克明 王宇光 |
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| 作者单位： | 1. 浙江科技学院,生物与化学工程学系,浙江,杭州,310012
2. 烟台大学,生物与化学理工学院,山东,烟台,264005 |
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| 摘 要： | 以玉米芯颗粒吸附桔青霉(Penicillium citrirum)孢子，再用1．5％的海藻酸钠包埋吸附固定化细胞玉米芯颗粒，于摇瓶中进行分批培养。实验结果表明：在固定化细胞产酶的条件下，培养基中淀粉水解糖浓度为9g／L，蛋白胨浓度为1g／L，摇瓶转速为180r／min，产酶发酵周期为50h，发酵液中核酸P1的活力高达503U／ml。双载体固定化细胞经30批次连续重复发酵产酶稳定在较高水平，固定化细胞粒子机械强度高。
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| 关 键 词： | 桔青霉 核酸酶P1 玉米芯 固定化细胞 海藻酸钠 淀粉水解糖 蛋白胨 发酵周期 活力 机械强度 稳定性试验 通气搅拌转速 |
| 文章编号： | 1671-8798(2003)02-0090-04 |
| 修稿时间： | 2002年12月6日 |
Study on nuclease P1 produced by immobilized Penicilium citrinum cell |
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| WANG Ke\|ming\,WANG Yu\|guang\.Study on nuclease P1 produced by immobilized Penicilium citrinum cell[J].Journal of Zhejiang University of Science and Technology,2003,15(2):90-93. |
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| Authors: | WANG Ke\|ming\ WANG Yu\|guang\ |
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| Institution: | WANG Ke\|ming\+1,WANG Yu\|guang\+2 |
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| Abstract: | Mycelia of \%Penicillium citrinum\% were absorbed with stone of maize and then wrapped up with 1.5% sodium alginate. The nuclease P\-1 produced by the immobilized cells were studied in shaking flasks. The experiment shows that the shaking speed is 180 r/min,the glucose and peptone contents in medium are 9 g/L and 1 g/L, respectly. After 50 h culture, the nuclease P\-1 activity reached 510 U/mL.In repeated batch culture, the immobilized cells kept the high capacity and machinery strength after 30 batches. |
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| Keywords: | penicillium citritum immobilized cell nuclease P\-1 stone of maize sodium alginate |
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