| 硫酸苯酚法与焦糖化分光光度法相结合测定藻类水溶液中总糖浓度 |
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| 引用本文： | 朱成文,白同春,刘德启,姚薇薇.硫酸苯酚法与焦糖化分光光度法相结合测定藻类水溶液中总糖浓度[J].苏州大学学报(医学版),2005,21(2):63-67. |
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| 作者姓名： | 朱成文 白同春 刘德启 姚薇薇 |
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| 作者单位： | 苏州大学化学化工学院，江苏苏州215006 |
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| 基金项目： | “十五”“211”工程重点学科建设子项目
上海市重点学科资助项目(W6-0339) |
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| 摘 要： | 藻在生长过程中分泌出的糖类物质的浓度比较低，并且随着藻的生长浓度的变化范围也比较大，通常单一的方法并不适应检测整个生长过程中的总糖浓度．本采用硫酸苯酚法与焦糖化分光光度法相结合的方法，测定含藻水溶液中总糖浓度的变化．从测定范围和准确性来看，均能满足测定的要求．
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| 关 键 词： | 分光光度法 糖浓度 水溶液 焦糖化 苯酚法 测定 硫酸 藻类 生长过程 糖类物质 生长浓度 浓度比 准确性 |
| 文章编号： | 1000-2073(2005)02-0063-05 |
| 修稿时间： | 2004年9月20日 |
Application of phenol-sulfuric acid method associated with caramelizing-grating photometer method to determine the content of polysaccharide in algae solution |
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| ZHOU Chen-wen,BAI Tong-chun,LIU De-qi,YAO Wei-wei.Application of phenol-sulfuric acid method associated with caramelizing-grating photometer method to determine the content of polysaccharide in algae solution[J].Journal of Suzhou University(Natural Science),2005,21(2):63-67. |
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| Authors: | ZHOU Chen-wen BAI Tong-chun LIU De-qi YAO Wei-wei |
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| Abstract: | The content of polysaccharide is an important index in purification of polysaccharide and environment evaluation. The methods of measuring the content of polysaccharide in algae solution are discussed in this paper. In this article, the phenol-sulfuric acid method combined with caramelization - grating photometer method is used to determine and monitor the content of sugar in algae solution. The result shows that this method is a simple and convenient method. The limit and sensitivity of this method and the application to different algae solution is discussed. |
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| Keywords: | algae polysaccharide phenol-sulfuric acid method caramelizing-grating photometer method |
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