| 番茄杂种一代与亲本的RAPD检测 |
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| 引用本文： | 恩和巴雅尔,邰丽华,王振兴,侯占铭.番茄杂种一代与亲本的RAPD检测[J].内蒙古师范大学学报(自然科学版),1999,28(4):325-326. |
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| 作者姓名： | 恩和巴雅尔 邰丽华 王振兴 侯占铭 |
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| 作者单位： | [1]内蒙古教育学院生物系 [2]内蒙古师范大学生物系 |
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| 摘 要： | 杂交种能够综合其双亲的许多优良性状,因此在生产上早已广泛应用.对于杂种一代的检测,目前主要还是靠田间观察,这不仅费时,也易受环境条件影响.90年代发展起来的随机扩增多态性DNA(Random Amplified Polymorphic DNA, 即RAPD)技术以其简便、灵敏和高效的优点,已开始在农作物杂交育种等相关研究领域得到应用1～4].本文根据RAPD技术的特点和实际结果,运用RAPD标记就番茄杂种与亲本的检测进行了讨论.
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| 关 键 词： | 番茄 杂种一代 亲本 RAPD检测 |
| 文章编号： | 1001-8735(1999)04-0325-02 |
| 修稿时间： | 1998-11-12 |
APPLICATION OF RAPD TO EXAMINATION AND DETERMINATION OF F1 AND PARENT OF TOMATO |
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| Enhebayer,TAI Li-hua,WANG Zhen-xing,HOU Zhan-ming.APPLICATION OF RAPD TO EXAMINATION AND DETERMINATION OF F1 AND PARENT OF TOMATO[J].Journal of Inner Mongolia Normal University(Natural Science Edition),1999,28(4):325-326. |
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| Authors: | Enhebayer TAI Li-hua WANG Zhen-xing HOU Zhan-ming |
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| Abstract: | DNA of
tomato was amplified by using 48 random primers in PCR were compared between the F\-1 and
parents.By 6 primers has existed polymorphism of DNA between parents can be determined.The
difference of parents DNA show codominance in F\-1.The result reveals a good application
potence of RAPD in examination and determination of F\-1 and parents of tomato |
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| Keywords: | tomato RAPD determinnation |
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