| 银杏叶中银杏内酯提取工艺研究 |
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| 引用本文： | 银杏叶中银杏内酯提取工艺研究.银杏叶中银杏内酯提取工艺研究[J].山东科学,2016,29(5):29-35. |
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| 作者姓名： | 银杏叶中银杏内酯提取工艺研究 |
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| 作者单位： | 1.山东农业大学食品科学与工程学院，山东 泰安 271018; 2.山东省中药质量控制技术重点实验室,山东省分析测试中心，山东 济南 250014;3. 济南森康三峰生物工程有限公司，山东 济南 250014 |
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| 基金项目： | 山东省科技发展计划(2014GZX219003) |
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| 摘 要： | 以银杏内酯(白果内酯、银杏内酯A、银杏内酯B和银杏内酯C)的提取率为指标，确定银杏叶乙醇提取的最佳提取工艺。采用正交试验设计的方法，系统考察提取时间、料液比、乙醇体积分数和提取次数4个因素对银杏叶提取工艺的影响。以异丙醇为流动相，优化高效液相色谱法(HPLC ELSD)测定银杏内酯的含量，并计算提取率。实验得出最佳提取工艺条件为提取时间1.5 h、料液比1：20、乙醇体积分数50%、提取次数3次。本工艺可以提高银杏内酯的提取率，节约试验成本，适用于工业化生产。
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| 关 键 词： | 异丙醇 正交试验 提取率 银杏内酯 银杏叶 |
| 收稿时间： | 2016-02-22 |
Extraction technology of ginkgolides from Ginkgo biloba leaves |
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| LI Wen dong,GAO Qian shan,YANG Peng,YANG Bing tian,WANG Xiao.Extraction technology of ginkgolides from Ginkgo biloba leaves[J].Shandong Science,2016,29(5):29-35. |
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| Authors: | LI Wen dong GAO Qian shan YANG Peng YANG Bing tian WANG Xiao |
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| Institution: | 1.School of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China; 2. Shandong Provincial Key Laboratory of Traditional Chinese Medicine Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China; 3. Jinan Senkang Sanfeng Biological Engineering Co. LTD, Jinan 250014, China |
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| Abstract: | We determined optimum extraction condition of Ginkgo biloba leaves with the extraction rate of ginkgolides (ginkgo lactone, ginkgolides A, ginkgolide B and ginkgolide C) as an index. We employed orthogonal experiment to systematically investigate the impact of extraction time, solid liquid ratio, ethanol concentration, and extraction time on extraction technology of Ginkgo biloba leaves. Ginkgolides content was determined through optimization of high performance liquid chromatography (HPLC ELSD) and isopropanol of mobile phase. We also calculated extraction rate. Optimum extraction conditions are extraction time of 1.5 h, solid liquid ratio of 1：20, ethanol concentration of 50% and extraction times of 3. This technology can improve extraction rate of ginkgolides, save testing cost, and is applicable to industrial production. |
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| Keywords: | extraction rate Ginkgo biloba leaves orthogonal experiment isopropyl alcohol ginkgolides |
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