功劳木中生物碱类成分抗氧化活性研究

利用DPPH法测定功劳木中生物碱成分的抗氧化活性，采用pH 区带精制逆流色谱分离出小檗碱、药根碱、巴马汀、非洲防己碱和千金藤宁碱5种成分。通过测定功劳木总生物碱以及单体生物碱成分对DPPH自由基清除力，来评价功劳木中主要生物碱类成分的体外抗氧化能力。结果显示，在0～25 μg/mL范围内DPPH溶液浓度与吸光度呈线性正相关关系。功劳木总生物碱、小檗碱、药根碱、巴马汀、非洲防己碱以及千金藤宁碱的半抑制浓度（IC50）依次为0.07、0.32、0.24、0.20、0.18、0.59 mg/mL。因此，功劳木生物碱类成分具有一定的抗氧化能力，且随生物碱浓度的升高而增强，总生物碱抗氧化能力强于单体成分。功劳木生物碱抗氧化活性可能为协同效应。

Antioxidant activity of alkaloids in Caulis Mahoniae

We determined antioxidant activity of alkaloids in Caulis Mahoniae by DPPH. We isolated berberine, jatrorrhizine, palmatine, columbamine and stepharanine with pH zone refining CCC. We also evaluated external antioxidant capability of dominant alkaloids in Caulis Mahoniae through DPPH free radical scavenging rate determination of total alkaloids and monomeric alkaloids. Results show that linear positive correlation exists for the concentration and absorbance of DPPH solution from 0 to 25 μg/mL. IC50 of total alkaloids, berberine, jatrorrhizine, palmatine, columbamine and stepharanine are respectively 0.07, 0.32, 0.24, 0.20, 0.18, and 0.59 mg/mL, so alkaloids of Caulis Mahoniae has certain antioxidant capability. It will be enhanced with the increase of alkaloids concentration. Moreover, antioxidant ability of total alkaloids is higher than that of monomeric compounds. Antioxidant activity of Caulis Mahoniae alkaloids may be synergistic effect.