| 一种大肠杆菌胆碱脱氢酶活性的测定方法 |
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| 引用本文： | 李佳忠,王春明,袁金铎,安利国.一种大肠杆菌胆碱脱氢酶活性的测定方法[J].山东科学,2006,19(1):32-34,49. |
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| 作者姓名： | 李佳忠 王春明 袁金铎 安利国 |
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| 作者单位： | 1. 山东师范大学生命科学学院,山东,济南,250014
2. 聊城大学生物系,山东,聊城,252000 |
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| 摘 要： | 本文提出了一种简单易行的测定微生物中胆碱脱氢酶活力的方法。通过测定549nm波长下电子传递体系PMS(吩嗪二甲酯硫酸盐)-cty.c(细胞色素C)光吸收值的增加,就可以计算出胆碱脱氢酶活力。利用此方法测定了大肠杆菌胆碱脱氢酶热激诱导前后的活力,结果发现受到诱导的大肠杆菌中胆碱脱氢酶的活力明显高于对照组。
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| 关 键 词： | 大肠杆菌 胆碱脱氢酶 酶的活力 酶的比活力 |
| 文章编号： | 1002-4026（2006）01-0032-03 |
| 收稿时间： | 2005-09-05 |
| 修稿时间： | 2005-09-05 |
A method for measuring the enzyme activity of E. coli Choline dehydrogenase |
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| LI Jia-zhong,WANG Chun-ming,YUAN Jin-duo,AN Li-guo.A method for measuring the enzyme activity of E. coli Choline dehydrogenase[J].Shandong Science,2006,19(1):32-34,49. |
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| Authors: | LI Jia-zhong WANG Chun-ming YUAN Jin-duo AN Li-guo |
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| Institution: | 1. School of Life Sciences, Shandong Normal University, Jinan 250014, China ; 2. Department of Biology, Liaocheng University, Liaocheng 252000, China |
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| Abstract: | This paper investigated a simple and available method for measuring the enzyme activity of Choline(dehydrogenase)(CDH) in microorganisms,it can be used as a parameter to estimate the synthesis of betaine in organisms.The(enzyme) activity of CDH can be calculated by measuring the absorption of cty.c at 549nm in electron transportation system PMS-cty.c.The result showed that the enzyme activity of the induced E.coli was higher than those of the control group's obviously. |
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| Keywords: | E coli Choline dehydrogenase enzyme activity specific activity |
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