无溶媒结晶技术在维生素B12生产中的应用

为了筛选无溶媒结晶工艺的最佳工艺参数，采用无溶媒结晶法制备维生素B12，以产品含量及收率为考察指标，用响应面分析法优化其制备工艺。得到最佳工艺参数：蒸发温度为65 ℃、真空度为0.09 kPa、结晶前溶液效价为90 000 μg/mL。实验结果表明，无溶媒结晶工艺稳定性较高、操作简单易行，制出的成品含量高、有关物质等质量指标较好，质量及收率整体水平不低于且部分批次优于溶媒结晶工艺。该技术在提高生产安全性的同时，大幅减少了对环境的污染，同时减少了相关工序，有效地降低了生产成本，适用于产业化生产。

Application of solvent-free crystallization technology in theproduction of vitamin B12

To prepare vitamin B₁₂ and to determine the optimal process parameters for the solvent-free crystallization technique, the purity and yield of the product were evaluated to optimize this technique using response surface methodology. The optimal process parameters were obtained as follows: evaporation temperature of 65 ℃, vacuum degree of 0.09 kPa, and concentration of 90 000 μg/mL prior to crystallization. It was shown that the solvent-free crystalization technique has high feasibility and effectiveness as the yield and quality of the final product were on par with that of solvent crystalization, if not better. While improving the safety of production, this technique cuts down pollution and eliminates certain procedures, thereby reducing the production cost and making it a good candidate for implementation in large-scale production.