HPLC法测定乙肝扶正清毒颗粒中芍药苷的含量

建立了采用高效液相色谱法测定乙肝扶正清毒颗粒中芍药苷含量的方法。采用 Agilent ZORBAX SB-C18色谱柱（4.6 mm×250 mm，5 μm），柱温25 ℃，以甲醇-0.05 mol/L磷酸二氢钾（体积比38:62）为流动相，进样量10 μL，流速1.0 mL/min，测定波长230 nm。 结果显示，芍药苷溶液在0.079 1～0.988 0 μg之间呈良好的线性关系(r=0.999 9)，加样回收率为98.1％~100.6％，相对标准偏差为1.02%(n=6)。本方法专属性强、灵敏度高、重现性好、操作简便，适用于乙肝扶正清毒颗粒的质量控制。

Content determination of paeoniflorin in Yiganfuzhengqingdu Granules by HPLC

A method for the determination of paeoniflorin content in Yiganfuzhengqingdu Granules by HPLC was established. HPLC analysis was carried out with Agilent ZORBAX SB-C18 chromatographic column (4.6 mm×250 mm，5 μm) and methanol-0.05 mol/L potassium dihydrogen phosphate(38:62, V/V) as mobile phase, the flow rate was 1.0 mL/min, and the column temperature was 25 ℃,injection volume was 10 μL, the detection wavelength was 230 nm. The results showed that the calibration curve was linear in the range of 0.079 1～0.988 0 μg for paeoniflorin (r=0.999 9), the recovery rate was 98.1%~100.6% with RSD 1.02%(n=6). This method is highly specific, sensitive, reproducible and simple, which can be used for quality control of Yiganfuzhengqingdu Granules.