| 壳聚糖酶高产菌株发酵条件的优化 |
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| 引用本文： | 郑岚,马耀宏,杨俊慧,张利群.壳聚糖酶高产菌株发酵条件的优化[J].山东科学,2010,23(5):10-14. |
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| 作者姓名： | 郑岚 马耀宏 杨俊慧 张利群 |
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| 作者单位： | 1. 山东轻工业学院
2. 山东省科学院生物研究所，山东省生物传感器重点实验室 |
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| 摘 要： | 优化了一株壳聚糖酶高产菌株的碳源、氮源、装瓶量、接种量和初始pH值等产酶发酵条件,并进行了正交实验。实验结果表明,最佳发酵条件为:几丁质2.5%,(NH4)2SO40.4%,酵母粉0.7%,pH值为5.5。优化后酶活由原来的454.03 U/mL提高至604.60 U/mL,提高量为优化前的33.16%。
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| 关 键 词： | 壳聚糖 壳寡糖 发酵条件 优化 |
| 收稿时间： | 2010-04-20 |
Optimization of Fermentation Conditions of Chitosanase High-Production Strain |
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| ZHENG Lan,MA Yao-hong,YANG Jun-hui,ZHANG Li-qun.Optimization of Fermentation Conditions of Chitosanase High-Production Strain[J].Shandong Science,2010,23(5):10-14. |
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| Authors: | ZHENG Lan MA Yao-hong YANG Jun-hui ZHANG Li-qun |
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| Institution: | 1. Shandong Institute of Light Industry
2. Shandong Provinceal Procincial Key Laboratory of Biosensors, Biology Institute, Shandong Academy of Sciences |
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| Abstract: | We optimized such fermentation conditions of a chitosanase high-production strain as carbon sources,nitrogen sources,bottling volume,inoculum size,initial pH value and performed an orthogonal experiment.Experimental results show that the optimum fermentation conditions are Chitin of 2.5%,(NH4)2SO2 of 0.4%,yeast extract of 0.7% and pH value of 5.5.Enzyme activity is increased from 454.03 U/mL to 604.60 U/mL(increment rate of 33.16%) after such optimization. |
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| Keywords: | chitosan chitosan oligosaccharide fermentation conditions optimization |
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