| 动物脑组织磷脂的薄层分离测定 |
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| 引用本文： | 杨雅婷,刘代成.动物脑组织磷脂的薄层分离测定[J].山东科学,2007,20(1):38-41. |
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| 作者姓名： | 杨雅婷 刘代成 |
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| 作者单位： | 山东师范大学生命科学学院,山东,济南,250014 |
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| 摘 要： | 以V(氯仿):V(无水乙醇):V(三乙胺):V(水)=10:11.3:11.7:2.7为展开剂,分别采用4种不同厂家生产的溴百里香酚蓝指示剂配制0.3%的溴百里香酚蓝水溶液(pH=7.0)作为显色剂,对牛脑组织中磷脂成分进行薄层层析分离和薄层扫描定量,该法分离、显色效果好,而且重现性、稳定性较好。
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| 关 键 词： | 脑组织 磷脂 薄层层析 薄层扫描 |
| 文章编号： | 1002-4026（2007）01-0038-04 |
| 收稿时间： | 2006-03-16 |
| 修稿时间： | 2006年3月16日 |
Separation and Determination of the Phospholipids from Animals' Brains by Thin-Lager Chromato Grnphy |
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| YANG Ya-ting,LIU Dai-cheng.Separation and Determination of the Phospholipids from Animals'''' Brains by Thin-Lager Chromato Grnphy[J].Shandong Science,2007,20(1):38-41. |
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| Authors: | YANG Ya-ting LIU Dai-cheng |
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| Institution: | School of Life Science, Shandong Normal. University, Jinan 250014, China |
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| Abstract: | The phospholipids from animals' brains were separated by one-dimensional thin-layer(chromatography) and determined by thin-layer chromatographic scanner.Chloroform-ethanol absolute-(triethylamine)-water(10:11.3:11.7:2.7) was used as developing solvent on an TLC silica plate of thin-lager chromatograph.Four different 0.3% Bromothymol Blue solutions from different companies were used for coloration of the samples.It can be concluded that this method is stable and repeatable. |
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| Keywords: | brain phospholipid thin-layer chromatography thin-layer scanning |
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