| 利用根癌农杆菌介导的转化方法改良木霉菌 |
| |
| 引用本文： | 黄玉杰,杨合同,陈凯,周红姿.利用根癌农杆菌介导的转化方法改良木霉菌[J].山东科学,2005,18(3):30-35. |
| |
| 作者姓名： | 黄玉杰 杨合同 陈凯 周红姿 |
| |
| 作者单位： | 1. 山东省科学院中日友好生物技术研究中心,济南,250014
2. 山东省科学院中日友好生物技术研究中心,济南,250014;山东理工大学生命学院,淄博,255049 |
| |
| 摘 要： | 根癌农杆菌介导的转化系统在丝状真菌的研究中具有重要的意义。通过农杆菌介导，成功实现了丝状真菌绿色木霉菌(Trichoderma viride)遗传转化，转化率约为30～80个转化子／10^5个孢子。PCR检测和几丁质酶分析表明含有编码几丁质酶外源基因(Cli 113)的T-DNA已整合进木霉菌基因组中，而且转化子都能够稳定遗传。农杆菌介导的遗传转化方法具有转化率高、操作简便、遗传稳定等优点，在丝状真菌的遗传转化中具有重要的意义。
|
| 关 键 词： | 根癌农杆菌 绿色木霉菌 转化 潮霉素抗性 生物防治 |
Modification of Trichoderma viride by Agrobacterium tumefaciens-mediated transformation |
| |
| HUANG Yu-jie,YANG He-tong,CHEN Kai,ZHOU Hong-zi.Modification of Trichoderma viride by Agrobacterium tumefaciens-mediated transformation[J].Shandong Science,2005,18(3):30-35. |
| |
| Authors: | HUANG Yu-jie YANG He-tong CHEN Kai ZHOU Hong-zi |
| |
| Institution: | HUANG Yu-jie 1,YANG He-tong 1,2*,CHEN Kai 1,ZHOU Hong-zi 1 |
| |
| Abstract: | Agrobacterium tumefaciens-mediated transformation (ATMT) system is a significant technical method in the study of many filamentous fungi. We successfully transformed the Trichoderma viride LTR-2 by A. tumefaciens mediated method with an efficiency of 30-80 transformants per 10 5 conidia. PCR analysis showed that only the T-DNA containing foreign gene chi113 encoding chitinase from Bacillus subtilis was integrated into the genome, and the foreign gene chi113 remained stably in transformants. The transformation system mediated by Agrobacterium tumefaciens may prove to be a powerful tool for the filamentous fungi transformation and functional genomic study with its high transformation frequency, simplicity of T-DNA integration, and genetic stability of transformants. |
| |
| Keywords: | Agrobacterium tumefaciens Trichoderma viride transformation Hygromycin resistance biocontrol |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
