| 超临界CO2萃取-高速逆流色谱分离纯化丹皮中丹皮酚 |
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| 引用本文： | 耿岩玲,刘建华,王岱杰,程传格,段文娟,王晓.超临界CO2萃取-高速逆流色谱分离纯化丹皮中丹皮酚[J].山东科学,2009,22(6):13-16. |
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| 作者姓名： | 耿岩玲 刘建华 王岱杰 程传格 段文娟 王晓 |
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| 作者单位： | 山东省分析测试中心,山东济南,250014 |
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| 基金项目： | 国家自然科学基金，山东省科技攻关项目 |
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| 摘 要： | 采用超临界CO2提取中药丹皮中总丹皮酚,选择萃取温度、萃取压力、物料粒度为因素进行正交实验优选出超临界CO2萃取的最佳工艺条件：即物料粒度40～60目,萃取温度40℃,萃取压力20MPa,提取率可达90%以上。将萃取后的总丹皮酚应用高速逆流色谱进行分离纯化,两相溶剂系统为石油醚-乙酸乙酯-甲醇-水（1：0.4：1：0.4）,进样量为330mg,得180mg纯度为99.0%的丹皮酚。
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| 关 键 词： | 丹皮 丹皮酚 超临界 高速逆流色谱 提取 分离纯化 |
Paeonol Extraction from Cortex Moutan with Supercritical Fluid Extraction and High-Speed Counter-Current Chromatography |
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| GENG Yan-ling,LIU Jian-hua,WANG Dai-jie,CHENG Chuan-ge,DUAN Wen-juan,WANG Xiao.Paeonol Extraction from Cortex Moutan with Supercritical Fluid Extraction and High-Speed Counter-Current Chromatography[J].Shandong Science,2009,22(6):13-16. |
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| Authors: | GENG Yan-ling LIU Jian-hua WANG Dai-jie CHENG Chuan-ge DUAN Wen-juan WANG Xiao |
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| Institution: | ( Shandong Analysis and Test Center, Jinan 250014, China) |
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| Abstract: | We employed supercritical fluid extraction （SFE） to extract paenonl from Cortex Moutan.We selected its optimum extracting condition from orthogonal experimental results.Results show that 40℃,20 MPa and a sample particle size of 40-60 mesh are the optimal condition. We also employed high-speed counter-current chromatography（HSCCC） to separate and purify Paenonl,in which the volume ratio of the composition of a two-phase solvent system was 1：0.4：1：0.4 （petroleum ether vs. ethyl acetate vs. methanol vs. water）. We further obtained paenonl （99.0% purity） 180 mg from crude extracts of 330 mg,as determined by HPLC. |
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| Keywords: | Cortex Moutan paenonl supercritical fluid extraction （SFE） high-speed counter-current chromatography extraction |
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