高速逆流色谱分离纯化灯盏细辛中的黄酮类化合物

采用高速逆流色谱技术快速分离纯化灯盏细辛中的黄酮类化合物,以石油醚-乙酸乙酯-甲醇-水(4:6:3.5:6.5,V/V)为溶剂系统,转速850 r/min,流速2.0 m L/min,检测波长254 nm。从500 mg粗提物中分离得到4个黄酮类化合物:黄芩素(87 mg)、木犀草素(35 mg)、槲皮素(46 mg)和芹菜素(92 mg),经高效液相色谱法分析,其纯度分别为94.6%、92.5%、98.2%、97.5%。研究结果表明,应用高速逆流色谱分离灯盏细辛中的黄酮类化合物快速、高效且纯度高。

Separation and purification of flavonoids from Erigeron breviscapus by high-speed counter-current chromatography

We rapidly separated and purified flavonoids from Erigeron breviscapus by high speed counter current chromatography (HSCCC). The extraction conditions were solvent system of petroleum ether ethyl acetate methanol water (4:6:3.5:6.5,V/V), rotation speed of 850 r/min,flow speed of 2 mL/min, and detection wavelength of 254 nm. Four flavonoids,baicalein (87 mg), luteolin (35 mg),quercetin (46 mg) and apigenin (92 mg) were separated from crude extract of 500 mg, whose purities are 94.6%, 92.5%, 98.2% and 97.5% by HPLC analysis. Experimental results show that the approach is rapid, highly efficient and purified.