不同产地丹参脂溶性成分对H9C2心肌细胞缺氧缺糖损伤保护作用的差异

通过HPLC谱图比较不同产地丹参脂溶性成分含量的差异,采用MTT染色法测定缺氧、缺糖1、2、3 h后H9C2心肌细胞的成活率以建立缺氧缺糖模型组,以低、中、高剂量的丹参脂溶性成分加入模型组细胞,观察细胞的状态确定加药浓度。检测各组的乳酸脱氢酶(LDH)含量、总抗氧化活力(T-AC)和细胞存活率(SR),以此作为考察丹参脂溶性成分保护作用差异的指标。结果表明,缺氧缺糖组以及不同产地丹参脂溶性成分治疗各组的SR与正常对照组相比有显著性差异(P0.05),不同产地丹参脂溶性成分治疗各组的SR与缺氧缺糖组相比有显著性差异(P0.05)。缺氧缺糖组以及不同产地丹参脂溶性成分治疗各组的LDH与正常对照组相比有显著性差异(P0.05),不同产地丹参脂溶性成分各组的LDH含量与缺氧缺糖组相比有显著性差异(P0.05)。缺氧缺糖组以及不同产地丹参脂溶性成分各组的T-AC与正常对照组相比有显著性差异(P0.05),不同产地丹参脂溶性成分各组的T-AC与缺氧缺糖组相比有显著性差异(P0.05)。丹参脂溶性成分明显减少心肌细胞缺氧缺糖损伤后LDH的溢出,提高了心肌细胞的T-AC和SR,其对缺氧缺糖损伤后心肌细胞确实有修复保护作用,不同产地丹参中脂溶性成分对心肌细胞缺氧缺糖保护作用确实存在药效差别。

Differences of protective effect of liposoluble components in Salvia miltiorrhiza Bge.from different origin places to H9C2 cardiac myocytes injured by oxygen/glucose deprivation

We compared content differences of liposoluble components of Salvia miltiorrhiza Bge.from different origin places with HPLC spectrogram.We determined the survival rate of cardiac myocytes injured by oxygen/glucose deprivation (OGD）for 1,2 and 3 h by MTT assay to construct the model group of oxygen/glucose deprivation. We further added low,medium and high dosage of liposoluble components in the cells of the model group and determined the concentration of drugs adding through the observation of cellular morphology.We then detected LDH content, T AC and survival rate (SR) of cells of every group, which were served as the parameters identifying the differences of protective effect of liposoluble components of Salvia miltiorrhiza Bge.Results show that the survival rates, LDH and T AC of OGD group and treatment group are significantly different from those of control group (P＜0.05).The survival rate, LDH and T AC of treatment group is also significantly different from those of OGD group(P＜0.05).Liposoluble components of Salvia miltiorrhiza Bge.dramatically reduce the overflow of LDH after H9C2 cardiac myocytes were injured through oxygen/glucose deprivation.They can also increase SR and T AC of cardiac myocytes,which do have protective effect to OGD damaged cardiac myocytes.Moreover, the protective effects of liposoluble components of Salvia miltiorrhiza Bge.from different origin places to H9C2 cardiac myocytes injured by OGD are indeed different.