基于UPLC方法同时测定准噶尔柳花中的芦丁、木犀草苷、绿原酸、槲皮素含量

利用UPLC法测定准噶尔柳花中的芦丁、木犀草苷、绿原酸、槲皮素含量。采用色谱柱：ACQUITY UPLC BEH C18 100 mm×2.1 mm，1.7 μm；流动相：乙腈（A） 0.2%乙酸水溶液（B），梯度洗脱，流速：0.29 mL/min；检测波长：360 nm；柱温：30 ℃。实验结果表明，芦丁线性范围0.05~3.12 μg/mL（r=0.999 8）,平均回收率为99.9%，相对标准偏差为1.6%；木犀草苷线性范围0.03~9.00 μg/mL（r=0.999 5）,平均回收率为98.8%，相对标准偏差为0.8%；绿原酸线性范围0.01~5.00 μg/mL（r=0.999 7）,平均回收率为97.6%，相对标准偏差为1.7%；槲皮素线性范围0.001~0.050 μg/mL（r=0.999 8）,平均回收率为99.0%，相对标准偏差为2.0%。该方法准确可靠，重复性好，可以作为准噶尔柳花的质量控制依据。

Based on the UPLC method for simultaneous determination of rutin,luteoloside,chlorogenic acid and quercetin from Salix songarica flower

A UPLC method for the determination of rutin, luteoloside, chlorogenic acid and quercetin from Salix songarica flower was established. The analysis was separated on ACQUITY UPLC BEH C18 (100 mm×2.1 mm, 1.7 μm) column, with acetonitrile (A) and water (containing 0.2% acetic acid, B), gradient elution. The flow rate was 0.29 mL/min and detected at 360 nm; the column temperature was 30 ℃. The linear range was 0.05~3.12 μg/mL (r=0.999 8), and the average recovery was 99.9%，with the RSD of 1.6% for rutin. The linear range was 0.03~9.00 μg/mL (r=0.999 5), and the average recovery was 98.8%，with the RSD of 0.8% for luteolin. The linear range was 0.01~5.00 μg/mL (r=0.999 7), and the average recovery was 97.6%，with the RSD of 1.7% for quercetin. The linear range was 0.001~0.05 μg/mL (r=0.999 8), and the average recovery was 99.0%，with the RSD of 2.0% for chlorogenic acid. This method was accurate and reliable, which can be regarded as a reference to control the quality of rutin, luteolin, quercetin and chlorogenic acid in the Salix songarica flower.