| 植物组织培养中消毒剂的运用 |
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| 引用本文： | 王利民,周毅,陈龙友,张宇斌.植物组织培养中消毒剂的运用[J].贵州师范大学学报(自然科学版),2002,20(1):15-17. |
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| 作者姓名： | 王利民 周毅 陈龙友 张宇斌 |
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| 作者单位： | 贵州师范大学生物科学技术系,贵州,贵阳,550001 |
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| 摘 要： | 对月季 (Roeschinensis)、香石竹 (Dianthuecaryophllus)、鱼腥草 (Houttuyniacordata)组织培养中几种常用消毒剂Ca(ClO) 2 、HgCl2 、CH3 CH2 OH、H2 O2 、漂白粉 (饱和溶液 )的运用进行了比较研究 ,结果表明 :0 1%HgCl2 消毒效果最好 ,污染率较低 ,但成活率也较低 ;用地下茎作材料进行组织培养时 ,75 %CH3 CH2 OH配合 0 1%HgCl2 效果较好
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| 关 键 词： | 植物组织培养 消毒剂 污染率 成活率 |
| 文章编号： | 1007-5570(2002)01-0015-03 |
| 修稿时间： | 2001年10月28 |
A study on the use of disinfectants in plant tissue culture |
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| WANG Li-min,ZHOU Yi,CHEN Long-you,ZHANG Yu-bin.A study on the use of disinfectants in plant tissue culture[J].Journal of Guizhou Normal University(Natural Sciences),2002,20(1):15-17. |
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| Authors: | WANG Li-min ZHOU Yi CHEN Long-you ZHANG Yu-bin |
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| Abstract: | This article compares the use of different disinfectants, Ca(CIO) 2,HgCI 2,H 2O 2, alcohol and saturate bleaching powder solution in tissue culture of Roes chinensis,Dianthue caryophllus and Houttuynia cordata. It has showed the disinfection effect of 0.1?% HgCl 2 is the best as the plan pollution rate is low. However, the survival rate is also low. When the underground stems are taken as tissue culture's material,it is more effective to use the 0.1% HgCl 2 with 10?% alcohol. |
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| Keywords: | plant tissue culur disinfectant pollution rate survival rate |
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