| 反相高效液相色谱法测定治咳枇杷露中乌索酸的含量 |
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| 引用本文： | 邹盛勤,刘红卫.反相高效液相色谱法测定治咳枇杷露中乌索酸的含量[J].贵州师范大学学报(自然科学版),2006,24(1):52-54,112. |
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| 作者姓名： | 邹盛勤 刘红卫 |
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| 作者单位： | 1. 宜春学院生物工程研究所,江西省天然药物活性成分研究重点实验室,江西,宜春,336000
2. 宜春学院,医学院,江西,宜春,336000 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 建立了反相高效液相色谱法测定枇杷露中乌索酸的含量,同时采用光电二极管阵列检测器检测乌索酸色谱峰的纯度。色谱柱为Nova-Pak C18(3.9×150 mm,4μm),流动相为甲醇-水(88∶12),流速1.0 mL/m in,检测波长210 nm,柱温25℃。乌索酸在0.039 5~0.237μg范围内线性关系良好(r=0.999 1),检出限为0.2 mg/L(3σ),平均回收率为98.7%。该法适于对含有乌索酸的药物进行质量评价。
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| 关 键 词： | 乌索酸 光电二极管阵列检测器 反相高效液相色谱 含量测定 |
| 文章编号： | 1004-5570（2006）01-0052-03 |
| 收稿时间： | 2005-08-26 |
| 修稿时间： | 2005-08-26 |
A determination of ursolic acid in Zhike Pipa Lu by RP-HPLC |
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| ZOU Sheng-qin,LIU Hong-wei.A determination of ursolic acid in Zhike Pipa Lu by RP-HPLC[J].Journal of Guizhou Normal University(Natural Sciences),2006,24(1):52-54,112. |
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| Authors: | ZOU Sheng-qin LIU Hong-wei |
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| Abstract: | A determination method of ursolic acid in Zhike Pipa Lu by reversed-phase high performance liquid chromtograph was established.At the time the purity of ursolic acid peak was detected by means of Photodiode Array Detector(PAD).The chromatographic column,Nova-Pak C18(3.9×150 mm,4 μm),methanol-water(88∶12,V/V) mobile phase with 1.0 mL/min flow rate,the detected wavelength(210 nm),and the column temperature(25 ℃) were adopted.The calibration curve of ursolic acid was linear under the content of 0.039 5～0.237 μg.The correlation coefficient was over 0.999 1.The detected limit was 0.2 mg/L(3σ).The average recovery of ursolic acid acid was 98.7 %.The method can be adapted to evaluate the quality of medicine that contains ursolic acid. |
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| Keywords: | ursolic acid photodiode array detector RP - HPLC content determination |
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