| 编码真核启动因子4E的毛尖紫萼藓基因GH425电子克隆及验证分析 |
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| 引用本文： | 沙伟,吴力.编码真核启动因子4E的毛尖紫萼藓基因GH425电子克隆及验证分析[J].贵州师范大学学报(自然科学版),2010,28(4). |
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| 作者姓名： | 沙伟 吴力 |
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| 基金项目： | 黑龙江科技厅农业攻关项目，黑龙江省教育厅海外学人合作项目，齐齐哈尔大学生命科学与农林学院遗传工程重点实验室基金支持 |
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| 摘 要： | GH425基因(genebank EST#:GPE204)来自毛尖紫萼藓干旱胁迫cDNA文库。本实验在小立碗藓核酸数据库中,以GH425基因为基因探针进行电子克隆,最终得到全长序列GH425NO.1。经ORFfinder分析,以最长ORF设计引物,对毛尖紫萼藓进行RT-PCR验证,得到了与之对应的条带,证明了毛尖紫萼藓中含有电子克隆的片段,即电子克隆结果正确。经Blastx分析,确定该序列编码真核启动因子4E。
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| 关 键 词： | 毛尖紫萼藓 eIF4E 抗旱 电子克隆 ORFfinder Blast |
In silicon cloning of GH425, a grimmia pilifera P.Beauv gene encoding a eukaryotic initiation factor 4E |
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| SHA Wei,WU Li.In silicon cloning of GH425, a grimmia pilifera P.Beauv gene encoding a eukaryotic initiation factor 4E[J].Journal of Guizhou Normal University(Natural Sciences),2010,28(4). |
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| Authors: | SHA Wei WU Li |
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| Abstract: | GH425 gene comes from the Grimmia pilifera P.Beauv drought stress of cDNA library.In this experiment,we have got the full sequence of GH425NO.1 by E-cloning which using GH425 as gene probe,in Physcomitrella patens DNA Datebase.Through using ORFfinder to find out the longest ORF and design primer for it,then,validated the Physcomitrella patens by PT-PCR,and we have obtained corresponding band and proved that the result of silicon cloning is correct and the fragment is contained in Grimmia pilifera P.Beauv.Now,we know the sequence encodes Eukaryotic initiation factor 4E by Blastx analyzing. |
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| Keywords: | Grimmia pilifera P Beauv eIF4E drought stress silicon cloning Blast |
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