| 刺梨的炮制及其炮制品中维生素C测定法 |
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| 引用本文： | 蔡晓静,潘丽丽,李慧娟,江帆.刺梨的炮制及其炮制品中维生素C测定法[J].贵州师范大学学报(自然科学版),2011,29(4):6-10. |
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| 作者姓名： | 蔡晓静 潘丽丽 李慧娟 江帆 |
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| 作者单位： | 贵州师范大学山地重点实验室分析测试中心,贵州贵阳,550001 |
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| 基金项目： | 贵州省科学技术基金项目(黔科合J字LKS[2009]22号) |
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| 摘 要： | 目的：以微波干燥技术探索刺梨炮制方法，并建立刺梨炮制品中维生素c的RP—HPLC含量测定方法。方法：以1％偏磷酸为提取溶剂，分析柱为迪马C18柱（4．6mm×250mm，5um），检测波长245nm，柱温：30℃；为流动相：0．1mol／L磷酸二氢钾-甲醇（97：3），流速1．0mL／min。结果：在79．24ug／m...
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| 关 键 词： | 刺梨 偏磷酸 反相高效液相色谱法 维生素C 含量测定 |
RP-HPLC determination of Vitamin C of Rosa roxburghii Tratt. |
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| CAI Xiao-jing,PAN Li-li,LI Hui-juan,JIANG fan.RP-HPLC determination of Vitamin C of Rosa roxburghii Tratt.[J].Journal of Guizhou Normal University(Natural Sciences),2011,29(4):6-10. |
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| Authors: | CAI Xiao-jing PAN Li-li LI Hui-juan JIANG fan |
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| Institution: | 1.Key Laboratory of Mountain Environment,Guizhou Normal University,Guiyang,Guizhou 550001; 2.School of Pharmacy,Guiyang Medical University,Guiyang,Guizhou 550004,China) |
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| Abstract: | Objective： To establish a RP-HPLC method for the determination of Vitamin C in the processed Roda roxburghii Tratt. Methods： The chromatographic conditions were as follows： Based on 1% partial phosphoric acid for extraction solvent , DiamonsilC-18 analytic chromatographic column （4. 6mm × 250mm, 5 um） ; The wavelength for detection was 245 nm; column temperature ： 30℃ ; The mobile phase consisted of 0. 1mol/L KH2PO4-methanol （97：3）, the follow rate was 1.0mL/ min. Results ： The linearity of the calibration curve was good over the range of 79.24ug/mL - 396.2g/ mL,the regression equation obtained was y =2 871 018. 141 7 - 18 443. 142 9 ,r =0. 999 9. the average recovery was 2. 775 %, with RSD of 2. 775 % （n = 9）. Conclusion： This method is sensitive, quick and accurate for the quality control of Rosa roxburghii Tratt. |
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| Keywords: | Rosa roxburghii Tratt RP-HPLC vitamin C assaying |
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