高效液相色谱同时测定儿童尿液中的地西泮和卡马西平

建立了新的高效液相色谱-紫外检测方法对两种抗癫痫药物地西泮(DP)和卡马西平(CBZ)进行同时测定。以乙腈-0.01mol/LKH2PO4(40∶60,v/v)为流动相,流速为0.4mL/min,紫外检测波长216nm时,使用Diam-oncil C18柱对两种药物能够达到很好的基线分离。两种药物的回收率在95%～104%之间,DP和CBZ的检测限分别为6ng/mL和15ng/mL。分别对0.5μg/mL的DP和CBZ进行了11次平行测定,其日内RSD对应为2.2%和1.4%。本方法灵敏、准确,已用于对儿童尿液中的DP和CBZ进行的定性及定量分析,并获得满意结果。

Simultaneous determination of diazepam and carbamazepine in children urine with high performance liquid chromatography

A new method was established for the simultaneous determination of diazepam(DP) and carbamazepine(CBZ) in human urine based on high performance liquid chromatography separation with UV detection.The determination was accomplished using a Diamoncil C18 column and a mobile phase containing acetonitrile-0.01mol/LKH2PO4(40:60,v/v).Under the optional situations,the precision,accuracy and linearity were satisfactory.In comparison with other methods,the HPLC method proved to be much more sensitive giving detection limits 6ng/mL of DP and 15ng/mL of CBZ.The average recoveries of the determination were 95%~104%.The relative standard deviations(RSDs) were 2.2%,1.4% for DP and CBZ,respectively.The developed method has been successfully used for the determination of the two drugs in children urine samples.