| 用紫外诱变及紫外+硫酸二乙酯复合诱变方法选育高产异淀粉酶菌株 |
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| 引用本文： | 王弋博,刘向阳,李三相,李勃,张海林.用紫外诱变及紫外+硫酸二乙酯复合诱变方法选育高产异淀粉酶菌株[J].青海大学学报,2003,21(4):7-10. |
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| 作者姓名： | 王弋博 刘向阳 李三相 李勃 张海林 |
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| 作者单位： | [1]天水师范学院，甘肃天水741000 [2]天水市中心血站，甘肃天水741000 |
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| 摘 要： | 以兰州市淀粉厂附近的土壤中分离到的地衣芽孢杆菌(Bacillus licheniformis)为出发菌株，经过紫外线诱变及紫外线(UV) 硫酸二乙酯(DES)复合诱变方法，从大量突变株中进行筛选，最终成功地选育出一株高产、稳定、中温的异淀粉酶生产菌株UV-6-DI，其产酶活力由出发菌的3．35U／mL。提高到7．37U／mL，提高了120％。
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| 关 键 词： | 紫外线 紫外线＋硫酸二乙酯 地衣芽孢杆菌 异淀粉酶 |
| 文章编号： | 1006-8996(2003)04-0007-04 |
Selection of isoamylase producing strain with UV and UV+DES |
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| WANG Yi-bo ,LIU Xiang-yang ,LI San-xiang ,LI Bo ,ZHANG Hai-lin.Selection of isoamylase producing strain with UV and UV+DES[J].Journal of Qinghai University(Natural Science),2003,21(4):7-10. |
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| Authors: | WANG Yi-bo LIU Xiang-yang LI San-xiang LI Bo ZHANG Hai-lin |
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| Institution: | WANG Yi-bo 1,LIU Xiang-yang 2,LI San-xiang 1,LI Bo 1,ZHANG Hai-lin 1 |
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| Abstract: | The Primitive strain was isolated from the soil of the starch factory in Lanzhou.After many UV and UV+DES mutagenetic treatments and selections from a lot of mutants..Finally we obtained a high yield,stable and mid-temperature strain which can yield isoamylase.The enzyme activity was increased from 3.35U/ml to 7.37U/ml(It was increased 120%). |
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| Keywords: | UV UV+DES Bacillas licheniformis isoamylase |
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