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从CIP引进马铃薯资源的倍性鉴定
引用本文:孙雪梅,王舰.从CIP引进马铃薯资源的倍性鉴定[J].青海大学学报,2007,25(1):62-64.
作者姓名:孙雪梅  王舰
作者单位:青海大学农林科学院,青海西宁810016
摘    要:以马铃薯根尖为材料,用对二氯苯饱和溶液、1%秋水仙碱以及冰水混合物进行顸处理,醋酸洋红和卡宝品红染色,比较不同预处理方法和染色液对制片效果的影响,结果表明,预处理采用1%秋水仙碱中期分裂相多,染色体分散,而染色液采用卡宝品红着色效果好;鉴定了从CIP引进的马铃薯材料60份,其中二倍体材料10份,三倍体材料7份,四倍体材料43份。

关 键 词:马铃薯  染色体  倍性
文章编号:1006-8996(2007)01-0062-03
修稿时间:2006-10-09

The study of potato ploidy identification
SUN Xue-mei,WANG Jian.The study of potato ploidy identification[J].Journal of Qinghai University(Natural Science),2007,25(1):62-64.
Authors:SUN Xue-mei  WANG Jian
Institution:Academy of Agriculture and Forestry Science, Qinghai University,Xining 810016, China
Abstract:To compare the infltmce of different pretreatment and coloration, the root tips of potato were pretreated with saturated paracide, 1% colchicines and glaciofluvial mixtute colouted with carbolic acid rubin and acetate rubin. The results showed that chromosome scattered relatively with 1% colchicines , coloration was distinct with carbolic acid rubin; 60 potato materials introduced from CIP was identified, which include 10 dipliod, 7 triploid and 43 tetralont.
Keywords:potato  chromosome  ploidy
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