| 黄粉虫抗冻蛋白基因TmAFP84的克隆和序列分析 |
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| 引用本文： | 邵强,闫清华,张文学,王兰,徐存拴.黄粉虫抗冻蛋白基因TmAFP84的克隆和序列分析[J].信阳师范学院学报(自然科学版),2006,19(3):290-293. |
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| 作者姓名： | 邵强 闫清华 张文学 王兰 徐存拴 |
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| 作者单位： | 河南师范大学,生命科学学院,河南,新乡,453007 |
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| 基金项目： | 河南省科技攻关资助项目(0224330110)，河南省动物学重点学科资助项目 |
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| 摘 要： | 对黄粉虫抗冻蛋白(AFPs)基因进行RT-PCR扩增后,构建克隆重组质粒pUCm-T-TmAFP84,经双酶切、PCR和测序鉴定,克隆出了与GenBank中公布的编码84个氨基酸基因(注册号AF159114)基本相一致的抗冻蛋白基因TmAFP84,其同源性高达98.81%.与其他编码84个氨基酸的抗冻蛋白基因的同源性为95.92%.进而构建了表达重组质粒pMAL-p2X-TmAFP84.
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| 关 键 词： | 黄粉虫 抗冻蛋白 RT-PCR 克隆 序列分析 |
| 文章编号： | 1003-0972（2006）03-0290-04 |
| 收稿时间： | 2005-05-19 |
| 修稿时间： | 2005年5月19日 |
Cloning and Sequence Analysis of Antifreeze Protein Gene TmAFP84 in the Beetle Tenebrio molitor |
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| SHAO Qiang,YAN Qing-hua,ZHANG Wen-xue,WANG Lan,XU Cun-shuan.Cloning and Sequence Analysis of Antifreeze Protein Gene TmAFP84 in the Beetle Tenebrio molitor[J].Journal of Xinyang Teachers College(Natural Science Edition),2006,19(3):290-293. |
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| Authors: | SHAO Qiang YAN Qing-hua ZHANG Wen-xue WANG Lan XU Cun-shuan |
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| Institution: | College of Life Sciences, Henan Normal University, Xinxiang 453007, China |
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| Abstract: | Tenebrio molitor antifreeze protein gene TmAFP84 was obtained through RT-PCR method.Then we constructed cloning recombinant plasmid pUCm-T-TmAFP84,which was identified by double restriction enzymes digestion analysis,PCR and sequencing.The sequence of TmAFP84 was compared with relative nucleotides reported in GenBank.Display: TmAFP84 shared high homology with other AFP gene of Tenebrio molitor,the value is up to 98.81%.We also constructed expressing recombinant vector pMAL-p2X-TmAFP84 for the future protein expression and research on the structure and the function. |
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| Keywords: | Tenebrio molitor antifreeze protein RT-PCR cloning sequence analysis |
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