| 人血清淀粉样蛋白A的原核表达及抑菌研究 |
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| 引用本文： | 徐世东,刘飞,阚云超,吴峻.人血清淀粉样蛋白A的原核表达及抑菌研究[J].信阳师范学院学报(自然科学版),2019(2):196-200. |
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| 作者姓名： | 徐世东 刘飞 阚云超 吴峻 |
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| 作者单位： | 南阳师范学院河南省伏牛山昆虫生物学重点实验室;上海市计划生育科学研究所国家人口计生委计划生殖基础重点实验室 |
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| 摘 要： | 人血清淀粉样蛋白A1(Serum amyloid A1 protein,SAA1)是由肝脏分泌的一种急性时相反应蛋白,在炎症、创伤或感染后在血浆中迅速上升.利用实验室前期构建的SAA1蛋白表达载体p ET28a-SAA1-β,原核表达重组人血清淀粉样蛋白A1的β亚基,纯化获得高纯度的SAA1-β蛋白.同时,将表达的SAA1-β蛋白与白色念珠菌SC5314共培养,检测其对SC5314生长状态的影响.结果显示,在蛋白浓度为0、2、20、200 mg/L时,菌落平均个数分别为423、385、262和217个,抑菌率分别是0%、8.98%、38.06%和48.70%,表明SAA1-β蛋白浓度在200 mg/L范围内,对白色念珠菌SC5314有较好的抑制作用,且抑制作用与蛋白浓度呈正相关.
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| 关 键 词： | 血清淀粉样蛋白A1 表达纯化 白色念珠菌SC5314 抑菌作用 |
Expression of Recombinant Human Serum Amyloid Protein and the Ability to Inhibit the Growth of Candida Albicans |
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| Institution: | ,Henan Provincial Key Laboratory of Funiu Moutain Insect Biology,Nanyang Normal University,Municipal Commission of Population and Family Planning Key Laboratory of Contraceptrives and Devices on State Population,Shanghai Institute of Planned Parenthood Research |
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| Abstract: | Serum amyloid A1 is an acute phase reaction protein secreted by the liver,which rises rapidly in plasma after inflammation,trauma or infection. The recombinant human serum amyloid A1-β subtype expressed by prokaryotic vector p ET28 a-SAA1-β. The SAA1-β protein was purified and identified by Western Blot. Meanwhile,SAA1-βprotein was co-cultured with Candida albicans SC5314 to detect its effect on the growth of SC5314. The results showed that when the protein concentration was 0、2、20、200 mg/L,the average number of colonies was 423,385,262 and217,and the bacteriostasis rates were 0%、8.98%、38.06% and 48.70%,respectively. The results showed that SAA1-β protein concentration in the range of 200 mg/L had a good inhibitory effect on Candida albicans SC5314,and the inhibition was positively correlated with the protein concentration. |
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| Keywords: | serum amyloid A1 expression and purification Candida albicans SC5314 bacteriostatic effect |
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