大狼把草内生真菌多样性研究

为了解我国入侵植物大狼把草(Bidens frondosa)内生真菌群落结构与分布规律,通过采样、组织分离、培养与鉴定,对大狼把草内生真菌多样性进行了分析.结果表明:大狼把草内生真菌种类丰富,有真菌19种.其中,产孢真菌有11属17种,全为半知菌,未产孢真菌2种,优势内生真菌为刺盘孢菌(Colletotrichumsp.)、拟茎点霉1(Phomopsissp.1)和链格孢菌(Alternariasp.).大狼把草内生真菌分布普遍,组织总定殖率为51.0%,其中,叶部定殖率大于茎部,幼茎定殖率小于老茎,从叶基至叶尖其组织内生真菌的定殖率逐渐递减.宿主植物生境与组织类型对内生真菌的组成与分布有重要影响.

Studies on Diversity of Endophytic Fungi in Bidens frondosa

In order to understand the community structure and distribution of endophytic fungi in the plants ofBidens frondosa, the diversity of endophytic fungi was analyzed by sampling, tissue isolation, culture and identification.The results showed that there were abundant endophytic fungi in the plants ofBidens frondosa.There were 19 species in the plants. Among them, there were 17 species in 11 genera of sporulation fungi, all of which are Deuteromycetes fungi and there were 2 species Sporogenous fungi. The dominant endophytic fungi wereColletotrichumsp.,Phomopsissp.1 andAlternariasp. The distribution of endophytic fungi ofBidens frondosawas widespread, and the total colonization rate was 51.0%. The fungus colonization rate of leaf was higher than that of stem, the fungus colonization rate of young stem was lower than that of old stem, and the fungi colonization rate of endophytic fungi gradually decreased from leaf base to leaf tip. Habitat and tissue types of host plants had important effects on the composition and distribution of endophytic fungi.